系统性红斑狼疮患者血清补体C1q水平与外周血共刺激分子表达的相关性研究  

Correlation studies of the serum level of complement Clq and the expression of stimulating molecules in peripheral blood of SLE patients

作　　者：何媛[1] 曾文兴 冷耀明[1] 

机构地区：[1]九江学院附属医院检验科,江西九江332000 [2]江西省宜春市人民医院检验科

出　　处：《临床医学》2016年第11期1-3,共3页Clinical Medicine

摘　　要：目的探讨系统性红斑狼疮(SLE)患者中血清补体C1q水平变化与外周血淋巴细胞(PBLCs)共刺激分子(CD80/CD86)的表达,以及它们之间的相关性。方法研究46例SLE患者,其中狼疮性肾炎(LN)19例,非LN 27例;另按照病情活动性分为活动期组(24例)和稳定期组(22例),并选取30例同期健康体检者为对照组。采用免疫透射比浊法检测血清C1q水平,流式细胞术检测PBLCs CD80及CD86的表达。结果 SLE组(LN组和非LN组)血清C1q水平明显低于对照组,PBLCs表面CD80和CD86的表达高于对照组(P<0.05),LN组C1q水平低于非LN组、PBLCs表面CD80和CD86的表达高于非LN组(P<0.05)。与稳定期组比较,活动期组SLE患者C1q水平降低,PBLCs表面CD80和CD86的表达升高(P<0.05)。SLE患者中C1q与CD80、CD86呈负相关。结论 C1q和CD80、CD86呈负相关,C1q、CD80/CD86共刺激分子与SLE的发生发展密切相关。Objective To investigate the level change of serum anti-complement protein C1 q and the expression of stimulating molecules CD80 / CD86 in peripheral blood lymphocytes（ PBLCs） and their correlation in patients with systemic lupus erythematosus（ SLE）. Methods Forty-six patients with SLE were studied,19 cases with lupus nephritis（ LN group） and 27 cases without LN（ non LN group）. According to the disease activity,the 46 patients with SLE were divided into activity group（ 24 cases） and stable group（ 22 cases）,then 30 cases of healthy physical examination were selected as the normal control group. The serum level of C1 q was determined by immune turbidimetric method,and the expression of CD80 and CD86 on PBMCs were determined by flow cytomety. Results In SLE groups（ including LN group and non LN group）,the serum level of C1 q was significantly lower than that in normal control group,but the expression of CD80 and CD86 on PBMCs were higher than those in normal control group（ P〈0. 05）. The serum level of C1 q in LN group was lower than that in non LN group,and the expression of CD80 and CD86 in PBMCs iof LN group were higher than those of non LN group（ P〈0. 05）. Compared with stable group,the serum level of C1 q was lower but the expression of CD80 and CD86 in PBMCs were higher in activity group（ P〈0. 05）. There was significant negative correlation of C1 q with CD80 or C1 q with CD86 in SLE patients. Conclusion C1 q and are negatively correlated with CD80 and CD86. C1 q and stimulating molecules CD80 / CD86 are closely correlated with occurrence and development of SLE.

关 键 词：系统性红斑狼疮 C1Q CD80 CD86 

分 类 号：R593.241[医药卫生—内科学] R446.1[医药卫生—临床医学]