兼顾免疫组化和电镜样品的戊二醛–多聚甲醛混合固定液的应用  

The Application of Glutaraldehyde and Paraform Mixed Fixative for Specimens Used for Immunohistochemistry and Transmission Electron Microscope Observation

作　　者：陶亚群 李贵苹[1] 易静[1] 杨洁[1] Tao Yaqun Li Guiping Yi Jing Yang Jie(Department of Biochemistry and Molecular Cell Biology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, Chin)

机构地区：[1]上海交通大学医学院生物化学与分子细胞生物学系,上海200025

出　　处：《中国细胞生物学学报》2016年第12期1518-1522,共5页Chinese Journal of Cell Biology

摘　　要：探寻兼顾同一实验动物用于免疫组化和电镜标本取材的灌注固定液中戊二醛–多聚甲醛的合适配比。小鼠分别用不同配比的固定液进行心脏灌注固定,对心肌、肾脏、肝脏进行电镜观察,并选择心肌组织进行免疫组化反应和免疫胶体金标记。结果发现,采用0.10%戊二醛+4%多聚甲醛混合液灌注的小鼠各脏器既能很好地保存其超微结构,又能保存某些抗原活性。The present study introduces a technical experience to figure out a suitable ratio of glutaraldehyde and paraform in the perfusing fixative for specimens used for both immunohistochemistry and transmission electron microscope （TEM） observation in the same experimental animal. The heart of mouse was perfused with different ratios of fixative respectively. The myocardium, kidney and liver were then observed by TEM for ultrastructural changes. In addition, the myocardial tissue was selected for immunohistochemical reaction and immune colloidal gold labeling. The results showed that 0.10% glutaraldehyde＋4% paraform mixture is suitable for preservation of the ultrastructure and the antigen activities.

关 键 词：固定液 戊二醛 多聚甲醛 透射电子显微镜 激光共聚焦显微镜 

分 类 号：Q-33[生物学]