一株木薯拮抗内生细菌CEB33的鉴定及田间防效初步测定  

Identification of One Endophytic Bacterium Strain CEB33 from Cassava and Preliminary Evaluation of It's Control Effect in Field

作　　者：陈奕鹏[1] 霍姗姗[2] 时涛[1] 李超萍[1] 杨扬[1] 蔡吉苗[1] 黄贵修[1] CHEN Yipeng HUO Shanshan SHI Tao LI Chaoping YANG Yang CAI Jimiao Huang Guixiu(Environment and Plant Protection Institute, CATAS/Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of A griculture/Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests/Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests, Haikou, Hainan 571101, China Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou, Hainan 571101, China)

机构地区：[1]中国热带农业科学院环境与植物保护研究所农业部热带作物有害生物综合治理重点实验室海南省热带农业有害生物监测与控制重点实验室海南省热带作物病虫害生物防治工程技术研究中心,海南海口571101 [2]中国热带农业科学院热带生物技术研究所,海南海口571101

出　　处：《热带作物学报》2016年第12期2404-2408,共5页Chinese Journal of Tropical Crops

基　　金：国家木薯现代产业技术体系建设项目(No.CARS-12-hnhgx)

摘　　要：以木薯细菌性萎蔫病病菌为靶标,从健康木薯组织中分离获得一株有较好抑菌作用的内生细菌。生理生化测定、形态观察及16S rDNA序列分析表明其为类芽孢杆菌属。拮抗试验表明该菌株对木薯细菌性萎蔫病菌、疫霉根腐病菌、棒孢霉叶斑病菌和胶孢炭疽病菌均有较强的抑制作用。田间防控试验表明该菌株对木薯细菌性萎蔫病有较好的防治效果,具有良好的开发前景。The pathogen of Cassava Bacterial Blight was used as indicator, and one endophytic bacterium strain CEB33 with the high antimicrobial activity was isolated from the health cassava tissue. Physiological and biochemical characteristics, morphlogical identification and 16 S r DNA sequence analysis indicated this strain belong to genus Paenibacillus. Based on the test results, the strain CEB33 showed the high antimicrobial activities against Xanthomonas axonopodis pv. manihotis, Phytophthora palmivora, Corynespora cassiicola and Colletotrichum gloeosporioides. This strain can obviously suppress Cassava bacterial blight in the field trail, and has good prospects in the future.

关 键 词：木薯 拮抗内生细菌 类芽孢杆菌属 

分 类 号：S435.33[农业科学—农业昆虫与害虫防治]