基于多种信号放大策略的电致化学发光生物传感器的研究进展  

Progress of electrochemiluminescence biosensor based on multiple signal amplification strategies

作　　者：王海军[1] 袁若[1] Wang Hai-jun Yuan Ruo(School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China)

机构地区：[1]西南大学化学化工学院,重庆400715

出　　处：《化学传感器》2016年第3期1-8,共8页Chemical Sensors

基　　金：国家自然科学基金资助项目(21575116;21275119;51473136)

摘　　要：电致化学发光(ECL)生物传感器技术,结合了ECL分析技术的高灵敏度和高可控性与生物识别的高特异性,能显著提高生物分子检测的灵敏度和选择性,在临床检验学等研究中展现出巨大的应用潜力。提高检测灵敏度一直都是ECL生物传感领域共同的追求目标。目前,研究者们通过引入具有优良性质的纳米材料、酶、DNA等生物放大技术,或研究寻找新型的、具有高发光效率的且生物兼容性好的ECL试剂,并改善发光试剂与共反应试剂的存在与作用方式等来提高ECL传感器的检测灵敏度,并取得了较好的效果。该文主要综述了近几年ECL生物传感器构建中常用的信号放大策略。Electrochemiluminescence （ECL） immunoassay technology, combined the high sensitivity and controlla- bility of ECL analysis technique with high specificity of biological recognition, can significantly improve the detection sensitivity and selectivity of biological molecules, which shows great potential in clinical laboratory science. Improving the detection sensitivity has become common goal in the construction of ECL biosensor. Currently, by introducing nano-materials with excellent properties, enzymes, DNA and other biological amplification technology, finding new ECL reagents with high luminous efficiency and improving reaction efficiency of co-reagents, researchers constructed a lot of ECL biosensors with high detection sensitivity. This paper mainly reviews some commonly used signal amplification strategies in the construction of ECL biosensors in recent years.

关 键 词：电致化学发光 生物传感器 信号放大策略 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]