表面纳米沟槽对成纤维细胞定向行为的影响  

Effect of nano-grooves on the alignment of fibroblasts

作　　者：崔垚[1] 安宇[1] 金桐宇 张帆[1] 何品刚[1] Cui Yao An Yu Jin Tong-yu Zhang Fan He Pin-gang(School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China)

机构地区：[1]华东师范大学化学与分子工程学院,上海200241

出　　处：《化学传感器》2016年第3期32-36,共5页Chemical Sensors

基　　金：国家自然科学基金资助项目(21405049)

摘　　要：在皮肤伤口愈合中,细胞的定向行为对加速伤口愈合起着非常重要的作用。该文采用纳米压印技术制备了线宽为200 nm、周期为400 nm及深度为200 nm的沟槽结构,用于模拟体内的细胞外基质。将成纤维细胞L929培养在纳米沟槽上,观察细胞的延长和铺展方向,对细胞的定向行为进行了定量分析。结果表明,L929细胞在纳米沟槽上具有良好的延长性,并能够沿着纳米沟槽的方向铺展,说明制备的纳米沟槽能够诱导成纤维细胞的定向行为,为皮肤伤口快速愈合的临床研究奠定了基础。The alignment behavior of cells plays a very important role in accelerating skin wound healing. In this paper, nano-imprinting technique was used to fabricate a nano-grooves structure with 200 nm in width of grooves, 400 nm in period and 200 nm in depth, simulating the extracellular matrix in vivo. L929 ceils were cultured on the nano-grooves, followed by the observation and quantitative analysis of cell elongation and spreading direction. The results showed that L929 ceUs could be elongated and spread along the direction of nano-grooves, thus it Could be concluded that the prepared nano-grooves could induce the alignment behavior of fibroblasts, which would lay the foundation for the rapid healing of skin wounds in clinical research.

关 键 词：纳米沟槽 成纤维细胞 定向行为 

分 类 号：R641[医药卫生—外科学]