GHR基因敲除香猪的鉴定及生长指标测定  

The Growth Performance Determination and Identification of GHR Gene Knockout Xiang Pigs

作　　者：陈伟[1,2,3,4] 许厚强[1,2,3] 喻昌毅 陈明飞[3] 肖伟[3] 张鸣[1,2,3] 杨洋[1,2,3] 

机构地区：[1]贵州大学高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵阳550025 [2]贵州省动物遗传育种与繁殖重点实验室,贵阳550025 [3]贵州大学动物科学学院,贵阳550025 [4]贵州大学生命科学学院,贵阳550025

出　　处：《基因组学与应用生物学》2016年第12期3358-3364,共7页Genomics and Applied Biology

基　　金：贵州省科技计划项目(黔科合平台[2013]4003);国家科技支撑计划(2015BAD03B02-3)共同资助

摘　　要：为获得纯合的GHR基因敲除香猪的种群,本研究以贵州从江香猪为母本,中国农业大学提供的GHR基因敲除猪(GHR(+/-))为父本杂交选育F1代,筛选出F1代阳性杂合子猪和阴性猪,再用F1代阳性杂合子猪进行扩繁得到F2代。测定F1代和F2代猪的生长指标,同时检测F1代和F2代阳性猪。实验结果表明F1代GHR基因敲除阳性猪(GHR+/+)和阴性猪(GHR+/-)生长指标差异均不显著(p>0.05)。F2代GHR基因敲除猪在3月龄时体长、体重存在显著差异(p<0.05),F2代4月龄阳性猪与阴性猪各项指标均差异显著(p<0.05)。测序结果显示F1阳性杂合子猪共11头,阴性猪15头;F2阳性纯合子猪共8头,阴性猪9头。In order to obtain the homozygous GHR gene knockout pig population, we selected hybridization breeding of F1 generation pig by Guizhou Congjiang pigs （GHR＋/＋） as the female parent and china agricultural university provide GHR gene knockout pig （GHR＋/-） as the male parent, then we obtained F2 homozygous GHR gene knockout pig （GHR-/-） by crossbreeding of F1 generation pigs tested positive （GHR＋/- male and GHR＋/- female pigs）. Afterwards, we detected the growth performance of the F 1 generation pigs and F2 generation pigs. The result showed that the growth performance of F1 generation pigs tested positive （GHR＋/-） had lower than F1 generation pigs tested negative （GHR＋/＋）, and no significant difference （p〉0.05）. For hybrid F2 pigs, body length and body weight of GHR-/- pig had lower than GHR＋/＋ pigs in three-month-old, significant difference （p〈0.05）. All the indexes of F2 generation GHR-/- pigs had lower than F2 generation GHR＋/＋ pigs in four-month-old, significant difference （p〈0.05）. DNA sequencing result showed that GHR＋/- and GHR＋/＋ of the hybrid F1 pigs have 11 and 15 respectively, GHR-/- and GHR＋/＋ of the hybrid F2 pigs have 8 and 9 respectively.

关 键 词：GHR基因突变香猪 生长指标 体重 

分 类 号：Q953[生物学—动物学]