温度变化对刺参(Apostichopus japonicus)补体AjC3 mRNA和MicroR137活性的影响  

Effect of Temperature Changes on Activity of Complement Ajc3 mRNA and MicroR-137 in Sea Cucumber (Apostichopus japonicus)

作　　者：杜晓丽[1] 邵洁[1] 许艺迪 张峰[1] 

机构地区：[1]大连海洋大学,农业部北方海水增养殖重点实验室,大连116023

出　　处：《基因组学与应用生物学》2016年第12期3380-3383,共4页Genomics and Applied Biology

基　　金：国家自然基金“刺参免疫相关因子研究”资助

摘　　要：本研究分析了温度对刺参AjC3 mRNA活性、MicroR-137表达的影响及MicroR-137和AjC3mRNA活性的相关性。研究结果表明不同温度下AjC3 mRNA和MicroR-137的表达量不同;低温对刺参的免疫反应有抑制作用;AjC3 mRNA在脂多糖刺激下呈现先升高后下降的趋势,而MicroR-137则是呈现先降低后升高的趋势。实验表明,温度影响AjC3 mRNA的表达,对MicroR-137的表达影响不明显;MicroR-137和AjC3 mRNA之间存在负相关性,可能调控AjC3 mRNA的表达。In this research, we studied the effect of temperature on AjC3 mRNA activity, MicroR-137 expression level, and correlation of activity between MicroR-137 and AjC3 mRNA in Apostichopus japonicus. The results showed that the expression value of AjC3 mRNA and MicroR-137 were different under different temperature; Low temperature would restrain the immune reaction ofApostichopus japonicus; The expression value of AjC3 mRNA increased at first and then decreased under lipopolysaccharide stimulation, while the expression value of MicroR-137 decreased at first and then increased. The experiment results showed that temperature had influence on the expression ofAjC3 mRNA, while the influence on MicroR-137 was not obvious; The expression of MicroR- 137 was inversely correlated with AjC3 mRNA and MicroR-137 might regulate the expression of AjC3 mRNA in immune response.

关 键 词：RNA提取 刺参 miRNA AjC3 MRNA 实时定量PCR 

分 类 号：S917.4[农业科学—水产科学]