包裹液态氟碳的脂质纳米粒的制备及术中评估射频消融边界的实验研究  

Intraoperative assessment on radiofrequency ablation margins using lipid nanoparticles with liquid perflurocarbons

作　　者：纪莉[1] 杨丽[2] 张世玉[2] 谢明星[1] JI Li YANG Li ZHANG Shiyu XIE Mingxing(Department of Ultrasound, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, Chin)

机构地区：[1]华中科技大学同济医学院附属协和医院超声影像科,武汉市430000 [2]南方医科大学南方医院药学部

出　　处：《临床超声医学杂志》2016年第12期793-797,共5页Journal of Clinical Ultrasound in Medicine

基　　金：国家自然科学基金重点项目(81530056)

摘　　要：目的制备一种热敏型纳米粒——包裹液态全氟己烷(PFH)的脂质纳米粒,探讨其术中评估射频消融边界的可行性。方法采用均质-乳匀法制备包裹PFH的纳米粒,Malvern激光粒度分析仪检测纳米粒粒径范围。水囊实验观察纳米粒热相变情况;将24块大小相同的离体猪肝分为两组,分别给予单纯射频消融、射频消融联合纳米粒处理,术中观察气化区的形成,并测量气化区及大体病理的大小,分析其相关性。结果制备的热敏型纳米粒平均粒径为(328.00±65.57)nm。体外加热实验显示,当温度达到78℃时,二维超声观察纳米粒回声较加热前明显增强;离体猪肝消融实验显示,加入纳米粒后射频消融产生的气化区更明显,且与大体病理所示坏死范围呈高度相关(r=0.996、0.949)。结论通过均质-乳匀法成功制备了纳米粒,其粒径小且均一,具有热敏相变潜能,有望成为术中实时监控、评估射频消融边界的新方法。Objective To explore the feasibility of a new type of thermal- sensitive nanoparticles（Nps） was being prepared and used to assess radio-frequency ablation margins intra-operatively.Methods The nanoparticles surrounded with the liquid perfluorohexane（PFH）was prepared using homogenization /emulsion method. The range of Nps diameter was taken measured using Malvin laser particle size analyzer,and the characteristics of nanoparticles thermal phase transition was observed with water sac experiment. 24 pcs of fresh pork liver in vitro were ablated with radio-frequency ablation or combined with nanoparticles. The whole procedure of gasification zone formation was observed and the area of this zone was measured,and compared with the damaged area from the pathologic sample.Results The thermal-sensitive nanoparticles with a mean particle diameter（328.00±65.57）nm was successfully prepared. From the heating experiment in vitro,when the temperature rise up to 78 ℃,the echo of microsphere was higher than that of pre-heating period with two-dimensional ultrasonography. For in-vitro liver experiment,the gasification zone induced to radio-frequency ablation combined with nanoparticles was more obvious than using radio-frequency ablation only.Besides,that presented a high correlation with pathologic necrosis range.Conclusion The nanoparticles preparation by means of homogenization/emulsion technique is potential to be thermal-sensitive in phase transition,and to be a new technique used to assess radio-frequency ablation margins intra-operatively.

关 键 词：热敏 纳米粒 射频消融 离体猪肝 

分 类 号：R-33[医药卫生] R312