化学发光在生化分析中的应用研究进展  

Recent Advance in Chemiluminescence Assay and Its Biochemical Applications

作　　者：刘萌[1] 王子月[1] 张春阳[1] LIU Meng WANG Zi-Yue ZHANG Chun-Yang(College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China)

机构地区：[1]山东师范大学化学化工与材料科学学院,济南250014

出　　处：《分析化学》2016年第12期1934-1941,共8页Chinese Journal of Analytical Chemistry

基　　金：国家杰出青年科学基金(No.21325523);科技部重点领域创新团队(No.2015RA4024)资助~~

摘　　要：化学发光分析是利用化学发光反应的发光现象,对化学发光物质由激发态跃迁回基态时发出的光信号进行测量的一种分析方法。化学发光分析具有无需外来光源、灵敏度高、操作方便、分析快速以及易于实现自动化等优点,可与其它分析技术联用,在临床检验、药物分析和环境监测等领域具有广泛应用。近年来,纳米材料、生物芯片及微流控技术的引入促进了化学发光分析技术的发展。本文综述了化学发光分析与高效液相色谱、毛细管电泳、量子点、微流控芯片和微阵列、以及滚环扩增、等温指数扩增和两级等温扩增联用技术的发展,介绍了化学发光分析技术在DNA、生物小分子、生物酶、蛋白质和金属离子检测中的应用研究进展,并展望了其发展趋势。Chemiluminescence （ CL） assay is able to measure the optical signal emitted from the CL reagents as a result of the transition from the excited state back to the ground state. CL assay has significant advantages such as no external light source, high sensitivity, convenient operation, rapid analysis and easy automation, and has wide applications in clinical test, drug analysis and environmental monitoring. Recently, the introduction of nanomaterials, biochip and microfluidic techniques promote the development of CL assay. In this review, we summarize the recent progress in CL assay with the integration of high performance liquid chromatography, capillary electrophoresis, quantum dots, microfluidic chips, microarrays, rolling circle amplification, isothermal exponential amplification, and two-stage isothermal amplification for the detection of DNA, small biological molecules, enzymes, proteins and metal ions. We also give a summary of its future directions and highlight its potential applications.

关 键 词：化学发光 超灵敏检测 生化分析 综述 

分 类 号：O657.3[理学—分析化学]