淡黄花百合花药及子房不定芽诱导培养基筛选  

Screening on Anther and Ovary Adventitious Bud Induction Medium of Lilium Sulphureum Baker

作　　者：刘伟[1,2] 常征[1,2] 丁长春[1,2] LIU Wei CHANG Zheng DING Changchun(School of Environment and Resources, Wenshan University, Wenshan Yunnan 663099, China Wenshan Biological Resources Development and Research Center ,Wenshan Yunnan 663099, China)

机构地区：[1]文山学院环境与资源学院,云南文山663099 [2]文山州生物资源开发与研究中心,云南文山663099

出　　处：《文山学院学报》2016年第6期1-3,共3页Journal of Wenshan University

基　　金：云南省教育厅科研基金项目"淡黄花百合开花生物学特性研究"(2013Y582)

摘　　要：以淡黄花百合的花药和子房为外植体,采用MS培养基为基础培养基,研究不同植物生长调节剂及蔗糖对花药和子房不定芽诱导的影响,筛选出最佳诱导培养基。研究表明:花药作为外植体没有获得愈伤组织和不定芽,子房能培养出愈伤组织和生长健壮的不定芽;诱导子房产生愈伤组织的最佳培养基为NAA 0.5 mg/L+6-BA 0.5 mg/L+蔗糖4 g/L;诱导子房产生不定芽的最佳培养基为NAA 0.6 mg/L+6-BA 0.5 mg/L+蔗糖4 g/L。With yellow flower lily of anther and ovary as explants, using MS medium as basic medium, different plant growth regulators and sucrose＇s influence on anther and ovary adventitious bud induction are studied and the best induction medium is selected. The results shows that the anther as explant callus and adventitious bud are not obtained, the ovary can cultivate callus and adventitious bud of robust growth; Ovary producing the best medium for callus induction is NAA0.5 mg/L ＋ 6- BA0.5 mg/L ＋ sucrose 4 g/L; ovary producing the best medium for adventitious bud induction is NAA0.6 mg/L ＋ 6- BA0.5 mg/L ＋ sucrose 4 g/L.

关 键 词：淡黄花百合 花药 子房 组织培养 

分 类 号：S628.29[农业科学—园艺学]