安子合剂对抗磷脂抗体阳性流产小鼠TLR4/MyD88/NF-κB信号通路的影响  

Effects of Anzi Mixture on TLR4/My D88/NF- κB Signaling Pathway of Antiphospholipid Antibodies Positive Abortive Mice

作　　者：柳静[1] 陆启滨[1] 

机构地区：[1]南京中医药大学附属医院妇科,南京210029

出　　处：《中国药房》2017年第1期31-35,共5页China Pharmacy

基　　金：江苏省中医药局国家中医临床研究基地开放课题(No.JD201507);江苏省中医药局中医药领军人才课题(No.LJ200910);江苏省中医院2014年度院级课题博士项目(No.Y14019)

摘　　要：目的:研究安子合剂对抗磷脂抗体(APA)阳性流产小鼠Toll样受体4(TLR4)/髓样分化因子88(My D88)/核因子κB(NF-κB)信号通路的影响,探讨其抗APA阳性流产作用机制。方法:将BALB/c小鼠(♀)随机分为空白对照组、模型组、阿司匹林组(阳性对照,0.019 5 g/kg)和安子合剂低、中、高剂量组(37.7、75.4、150.8 g/kg,以生药计),每组10只。除空白对照组外,其余各组小鼠均以人β2-糖蛋白Ⅰ为诱导剂建立APA阳性流产模型。从妊娠第1天起,各给药组小鼠ig相应药物,空白对照组和模型组小鼠ig等体积生理盐水,每天1次,连续9 d。分别采用实时荧光定量聚合酶链反应法和免疫组化法测定胎盘组织TLR4、髓样分化蛋白2(MD2)、My D88、NF-κB m RNA及其蛋白表达水平。结果:与空白对照组比较,模型组小鼠胎盘组织TLR4、MD2、My D88、NF-κB m RNA及其蛋白表达水平均显著升高(P<0.01)。与模型组比较,阿司匹林组和安子合剂低、中剂量组小鼠胎盘组织TLR4、MD2、My D88 m RNA及其蛋白表达水平,安子合剂高剂量组小鼠胎盘组织TLR4蛋白表达水平,以及各给药组小鼠胎盘组织NF-κB蛋白表达水平均显著降低(P<0.05或P<0.01);安子合剂低剂量组小鼠胎盘组织TLR4、MD2 m RNA表达水平和MD2、My D88蛋白表达水平较阿司匹林组更低(P<0.05或P<0.01)。结论:安子合剂可抑制APA阳性流产小鼠TLR4/My D88/NF-κB信号通路转导,这可能是其抗APA阳性流产的作用机制之一。OBJECTIVE：To study the effects of Anzi mixture on Toll like receptor 4（TLR4）/myeloid differentiation factor 88（My D88）/nuclear facter-κB（NF-κB）signaling pathway of antiphospholipid antibodies（APA）positive abortive mice,and to investigate the mechanism of anti-APA positive abortion. METHODS：BALB/c mice（female）were randomly divided into blank control group,model group,aspirin group（positive control,0.019 5 g/kg） and Anzi mixture low- dose,medium- dose and high- dose groups（37.7,75.4,150.8 g/kg,calculated by crude drug）,with 10 mice in each group. Except for blank control group,other groups were given human β2-glycoprotein Ⅰ as derivant to establish APA positive abortion model. From the first day of pregnancy,treatment groups were given relevant medicine intragastrically,and blank control group and model group were given constant volume of normal saline intragastrically,once a day,for consecutive 9 d. m RNA and protein levels of TLR4,myeloid differentiation2（MD2）,My D88 and NF-κB in placental tissue of mice were determined by RT-PCR and immunohistochemical method. RESULTS：Compared with blank control group,m RNA and protein expression of TLR4,MD2,My D88 and NF-κB in placental tissue were increased markedly in the model group（P〈0.01）. Compared with model group,m RNA and protein expression of TLR4,MD2 and My D88 in aspirin group and Anzi mixture low-dose and medium-dose groups were decreased significantly as well as the protein expression of TLR4 in Anzi mixture high-dose group and the protein expression of NF-κB in all medicine groups（P〈0.05 or P〈0.01）. m RNA expression of TLR4 and MD2 and the protein expression of MD2 and My D88 in Anzi mixture low- dose groups were lower than those in aspirin group（P〈0.05 or P〈0.01）. CONCLUSIONS：Anzi mixture can inhibit TLR4/My D88/NF-κB signaling pathway of APA positive abortive mice,which may be one of anti-APA positive abortion mechanisms.

关 键 词：安子合剂 抗磷脂抗体 TLR4/My D88/NF-κB信号通路 流产小鼠 胎盘组织 

分 类 号：R285.5[医药卫生—中药学]