中药肝豆汤含药血清对Wilson病模型TX小鼠肝细胞内ATP7b蛋白亚细胞定位和功能表达的影响  

Effects of Chinese medicine Gandou Decoction on the ATP7b protein subcellular location and expression in the hepatocytes of TX mice with Wilson disease model

作　　者：徐陈陈 董健健[2] 程楠[2] 韩咏竹[2] 王训[2] 

机构地区：[1]安徽中医药大学研究生部,合肥230038 [2]安徽中医药大学神经病学研究所附属医院神经内科,合肥230061

出　　处：《中华中医药杂志》2017年第1期250-253,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81573954;No.81473535)~~

摘　　要：目的:观察肝豆汤调控TX小鼠肝细胞内铜转运通路的分子靶点,研究其调控机制。方法:免疫荧光双标记经激光共聚焦显微镜观察由含肝豆汤兔血清孵育后TX小鼠肝细胞内ATP7b蛋白的亚细胞定位变化。结果:ATP7b蛋白在DL小鼠肝细胞内定位于反面高尔基体管网状结构(TGN),位于高铜环境中则转运至顶端膜区,去除高铜环境则重新定位于TGN;ATP7b蛋白在TX小鼠肝细胞内不能定位于TGN及顶端膜区域,去除高铜环境也不能促使ATP7b蛋白重新定位于TGN。中药肝豆汤可促使ATP7b蛋白逐步重新定位于TGN。结论:肝豆汤可以通过诱导ATP7b蛋白重新定位于TGN,进而发挥细胞内排铜作用,改善Wilson病的临床症状。Objective：To observe the molecular target that Gandou Decoction regulates on the pathway of copper transportation in the hepatocytes of toxic milk（TX） mice and study the mechanism of regulation.Methods：By the method of immunofluorescent double-labeling,it was observed that the ATP7 b protein subcellular location in the TX mouse hepatocytes incubated in rabbit serum with Gandou Decoction.Results：The ATP7 b protein was located in the trans Golgi network（TGN）in hepatocytes of DL mice.When the hepatocytes were in the environment of high-concentration cupricion,the ATP7 b was transferred to the apical region and returned to the TGN while off that environment.The ATP7 b protein in hepatocytes of TX mice was neither located in the TGN,nor in the apical region even given the same environment as above.Gandou Decoction could stimulate the translocation of ATP7 b protein of TX mice from the cytoplasm to the TGN.Conclusion：Gandou Decoction could stimulate the translocation of ATP7 b protein to TGN in the hepatocytes of TX mice,and further to expel the cupricion,which may relieve the symptoms of Wilson disease.

关 键 词：肝豆汤 TX小鼠 WILSON病 ATP7b蛋白 反面高尔基体管网状结构 免疫荧光双标记 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]