黄芪甲苷对链脲佐菌素诱导糖尿病大鼠肾脏保护机制探讨  

Effects of astragaloside Ⅳ on renal damage in streptozotocin STZ-induced diabetic rats

作　　者：范愈燕[1,2] 吕翠岩[3] 朱斌[1] 李平[4] 刘庆山 刘延青[1] 

机构地区：[1]首都医科大学附属天坛医院,北京100050 [2]首都医科大学附属北京同仁医院,北京100005 [3]首都医科大学附属北京中医院,北京100730 [4]中日友好医院临床医学研究所,北京100029 [5]中国少数民族传统医学研究院,北京100030

出　　处：《中华中医药杂志》2017年第1期294-298,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81141040)~~

摘　　要：目的:观察黄芪甲苷对链脲佐菌素(STZ)诱导糖尿病大鼠肾脏保护机制。方法:4周龄Waster大鼠除了正常组,给予2周高脂肪饮食及链脲佐菌素诱导2型糖尿病成膜后随机分组:模型组、黄芪甲苷组(10mg/kg)、奥美沙坦组(100mg·kg^(-1)·d^(-1))及四甲基哌啶组(3mol/L),6周治疗观察血压及肾脏组织改变。结果:STZ诱导糖尿病12周龄的大鼠血压、血糖、尿蛋白、尿肌酐清除率及肾皮质纤维化基因均显著升高,升高的数值显著被奥美沙坦所抑制,而黄芪甲苷和四甲基哌啶均抑制除了血压以外的异常指标。模型组的肾皮质AGT及renin基因异常升高,除了奥美沙坦组明显改善外,其他组均无显著改变。结论:黄芪甲苷对STZ诱导糖尿病肾脏保护作用机制并未参与肾内血管紧张素系统,可能是通过抗氧化作用实现的。Objective：To investigate the effect of astragaloside Ⅳ on renal damage in STZ-induced diabetic rats.Methods：four-week old Waster rats were randomly divided into 5 groups：the control group（n=6）,model group（n=10）,astragaloside Ⅳgroup（10mg/kg,gavage;n=8）,olmesartan group（100 mg·kg-1·d-1,gavage;n=8） and tempol group（3mol/L;n=10）,the model of type 2 diabetes was established by STZ injection,all the rats received high fat diet for 2 weeks except the control.The renal function and fat metabolism were observed in 6-week treatment.Results：The blood glucose,urinary protein and creatinine clearance rate were significantly increased in STZ-induced diabetic 12-week old rats,while these changes were significantly reversed by olmesartan,astragaloside Ⅳ and tempol could inhibit above changes expect blood glucose.The gene expression of renal cortex renin and angiotensinogen in diabetic rats were significant increased,which were reversed only in olmesartan group.Conclusion：The renal protections of astragaloside Ⅳ on STZ-induced diabetes may be involved in antioxidant action.

关 键 词：2型糖尿病 黄芪甲苷 血管紧张素系统 抗氧化作用 大鼠 

分 类 号：R285.5[医药卫生—中药学]