金匮肾气丸对微波辐射性生殖功能障碍小鼠生精细胞Ca^(2+)、RyRs及MMP的影响  

Effects of Jinkui Shenqi Pills on Ca^(2+),RyRs and MMP of spermatogenic cells in mice with reproductive dysfunction induced by microwave irradiation

作　　者：毕焕洲[1] 彭超[1] 徐世杰[1] 

机构地区：[1]大连大学附属中山医院,大连116001

出　　处：《中华中医药杂志》2017年第1期332-334,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81573975)~~

摘　　要：目的:探讨微波辐射对小鼠生精细胞损伤机制及金匮肾气丸治疗机制。方法:将50只雄性BALB/c小鼠随机分为正常组,辐射组,金匮1、2、3组。除正常组外,其余4组均暴露于2 450MHz、10m W/cm2连续微波中,1h/d,18d。金匮1、2、3组每次分别给予0.1、0.2、0.4g/kg体质量金匮肾气丸灌胃,正常组、辐射组分别给予0.1m L 0.9%氯化钠溶液灌胃,1次/d,18d。检测小鼠生精细胞内Ca^(2+)荧光强度、内质网钙通道蛋白(Ry Rs)、线粒体膜电位(MMP)。结果:与正常组比较,辐射组Ca^(2+)荧光强度显著升高,而MMP、Ry Rs阳性表达显著降低(P<0.05);而金匮1、2、3组与辐射组比较,Ca^(2+)荧光强度降低,MMP、Ry Rs阳性表达显著升高(P<0.05),呈浓度依赖型,其中,金匮3组在Ca^(2+)荧光强度方面改善优于金匮1、2组(P<0.05)。结论:微波辐射损伤可使生精细胞内Ca^(2+)浓度增高,MMP、Ry Rs阳性表达降低,金匮肾气丸可通过调节Ry Rs、MMP修复损伤,降低细胞内Ca^(2+)浓度而达到治疗效果,且药量增加效果显著。Objective：To explore the mechanism of microwave irradiation on the damage of spermatogenic cells in mice and the therapeutic mechanism of Jinkui Shenqi Pills.Methods：Fifty male BALB/c mice were randomly divided into normal group,irradiation group,Jinkui group 1,Jinkui group 2,and Jinkui group 3.In addition to the normal group,the other 4 groups were exposed to 2 450 MHz,10mW/cm^2 continuous microwave one hour per day for 18 days.Mice in Jinkui group 1,Jinkui group 2,and Jinkui group 3 received gavage administration with 0.1,0.2,0.4g/kg Jinkui Shenqi Pill respectively,and mice in normal group and irradiation group received gavage administration with 0.1 mL of 0.9%sodium chloride solution once a day for 18 days.The fluorescence intensity of Ca^（2＋） in spermatogenic cells,ryanodine receptors（RyRs）,and mitochondrial membrane potential（MMP）were detected.Results：Compared with the normal group,the fluorescence in tensity of Ca^（2＋） in irradiation group was significal rising while the number of MMP,RyRs positive cells was significal reduced,and the fluorescence intensity of Ca^（2＋） in Jinkui group 1,Jinkui group 2 and Jinkui group 3 were decreasing,the number of RyRs positive cells,and MMP were significal increasing（P〈0.05）,and there were concentration dependence.Compared with Jinkui group 1 and Jinkui group 2,Jinkui group 3 improved significantly in the improving Ca^（2＋） intensity department（P0.05）.Conclusion：Microwave radiation damage can increase Ca^（2＋） concentration in spermatogenic cell,and Jinkui Shenqi Pills can repair the damage by regulating RyRs and MMP,and the therapeutic effect increases with the increase of the dose.

关 键 词：微波辐射 男性生殖功能障碍 生精细胞 钙离子 内质网钙通道蛋白 膜电位 金匮肾气丸 

分 类 号：R285.5[医药卫生—中药学]