应用亚临界水技术对枇杷叶中熊果酸的提取工艺优化研究  

Optimization research of extraction process of ursolic acid from loquat leaf with subcritical water technology

作　　者：秦霞[1] 刘红[1] 任晓燕[1] 柴翠元[1] QIN Xia LIU Hong REN Xiaoyan CHAI Cuiyuan(Department of Chemistry, Huainan United University,Huainan 232038,Chin)

机构地区：[1]淮南联合大学化工系,安徽淮南232038

出　　处：《黑龙江工程学院学报》2016年第6期41-44,共4页Journal of Heilongjiang Institute of Technology

基　　金：安徽高校省级自然科学研究项目(KJ2014A235);2014安徽省高校优秀青年人才支持计划;安徽省高校青年人才基金重点项目(2013SQRL139ZD);高校省级自然科学研究重点项目(KJ2015A430;KJ2016A655)

摘　　要：因实用的药用价值,熊果酸的亚临界水萃取技术受到相关研究者的关注。为有效提高熊果酸的提取率,以亚临界水萃取过程中的萃取时间、萃取温度、溶剂流速和系统压力为可变因素,通过设计正交试验及应用高效液相色谱对熊果酸进行定量测定,最终确定最佳的提取工艺:原料的粒径60目、提取温度200℃、提取时间75min、系统压力15 MPa,提取率达到70.09%。亚临界水萃取方法不仅提高熊果酸的提取率,还是一种新型的绿色萃取技术,具有良好的发展前景。The subcritical water extraction technology of bear acid is concerned by the researchers because of its practical medicinal value.In order to improve the extraction rate of the ursolic acid,this paper takes extraction time,extraction temperature,solvent flow rate and system pressure in the process of subcritical water extraction as variable factors,by designing orthogonal test and using high performance liquid chromatography to quantitative analysis ursolic acid,and finally determines the best extraction process：particle size 60 mesh of raw material,extraction temperature of 200℃,extraction time of 75 min,system pressure of 15 MPa,and extraction rate reached 70.09%.The subcritical water extraction method not only improves the extraction rate of the ursolic acid,but also serves as a new green extraction technology with development prospects.

关 键 词：亚临界水提取 枇杷叶 熊果酸 高效液相色谱 

分 类 号：O636[理学—高分子化学]