甘蓝AP2/ERF转录因子的克隆和生物信息学分析  

In Silico Cloning and Bioinformatics Analysis of AP2/ERF Family Gene from Brassica Oleracea

作　　者：郭慧[1] 金司阳 刘寒[1] 王振月[1] 

机构地区：[1]黑龙江中医药大学,哈尔滨150040

出　　处：《中国药师》2017年第1期6-10,共5页China Pharmacist

基　　金：国家自然科学基金项目(编号:30970300);哈尔滨科技创新人才研究专项基金项目(编号:2010rfxxs031)

摘　　要：目的:运用电子克隆的方法获得甘蓝中AP2/ERF转录因子基因。方法:以拟南芥的AP2/ERF转录因子作为探针,对甘蓝的EST数据库进行搜索,应用相关软件进行序列拼接组装和延长。结果:克隆出甘蓝两个AP2/ERF-B3亚族转录因子Bo AP2/ERF1和Bo AP2/ERF2。通过生物信息学方法,对两条序列编码蛋白质的氨基酸组成,亲水性/疏水性、理化性质、亚细胞定位、高级结构和空间构型等方面进行了预测和分析。结论:Bo AP2/ERF1和Bo AP2/ERF2基因由371和352个氨基酸组成、相对分子质量为40.85k Da和39.44k Da的蛋白质,定位于细胞核。序列分析结果显示,该蛋白可能具有信号转导和胁迫响应应答等功能。Objective： To obtain AP2/ERF genes from Brassica oleracea by using in silico cloning. Methods： AP2/ERF genes were cloned by retnieving the EST database and using the bioinformatics software with the Arabidopsis thaliana AP2/ERF as a querying probe. Results ： Two AP2/ERF family transcriptional regulators （ BoAP2/ERF1 and BoAP2/ERF2） were isolated from Brassica olera- cea by the in silico cloning method. Some characters of AP2/ERF protein were analyzed and predicted by the tools of bioinformatics in the following aspects including the composition of amino acid sequence, hydrophilicity and hydrophobicity, subcellular localization, secondary and tertiary structure of protein and function. Conclusion： Bioinformatical analysis shows BoAP2/ERF1 and BoAP2/ERF2 gene encode 40.85kDa and 39.44kDa protein with 371 and 352 amino acids. The domain is predicted to locate on nucleus. Sequence analysis indicates the protein may be involved in signaling transducer and stressing response roles in plantbiotic stresses.

关 键 词：电子克隆 甘蓝 AP2/ERF基因 生物信息学 

分 类 号：Q943.2[生物学—植物学]