HPLC法测定泮托拉唑钠生物黏附片的含量  

Determination of Pantoprazole Sodium Bioadhesive Tablets by HPLC

作　　者：马宁[1] 蒋瑶[1,2] 刘卫平[1] 曹伯进 褚燕斌 王建芬[1] 

机构地区：[1]长沙医学院药学院,长沙410219 [2]广东药科大学药科学院 [3]湖南九典制药股份有限公司

出　　处：《中国药师》2017年第1期161-163,共3页China Pharmacist

基　　金：湖南省科技厅科学计划项目(编号:2015SK20736);2014年省级药学类专业校企合作人才培养示范基地项目(编号:湘教通[2014]272号)

摘　　要：目的:建立HPLC法测定泮托拉唑钠生物黏附片的含量。方法:采用高效液相色谱法。色谱柱:HYPERSILODS-2(150 mm×4.6 mm,5μm);流动相:0.01 mol·L^(-1)磷酸氢二钾溶液(用磷酸调p H=7.0)-甲醇(60∶40);流速:1.0 ml·min^(-1);检测波长289 nm;柱温30℃;进样量:20μl。结果:泮托拉唑钠线性范围为1.28~20.60μg·ml^(-1),r=0.999 8,加样回收率为99.52%(RSD=1.43%,n=9)。结论:该方法简便、准确可靠,可用于泮托拉唑钠生物黏附片的含量测定。Objective： To establish a method to determine the contents of pantoprazole sodium bioadhesive tablets. Methods： An HPLC method was adopted. The determination was performed on a HYPERSIL ODS-2 （ 150 mm × 4.6 mm, 5 μm） column with mobile phase consisting of 0.01 mol L-1 dipotassium phosphate solution - methanol （60：40） at the fl0w rate of 1.0 ml min-l The de- tection wavelength was set at 289 nm, the column temperature was maintained at 30 ℃ and the sample size was 20 μl. Results： The linear range of pantoprazole sodium was 1.28-20.60 μg ml - 1, （ r = 0. 999 8 ） . The average recovery was 99.52% （ RSD = 1.43% , n = 9）. Conclusion： The method is simple, accurate and reliable, and can be used for determining the content of pantoprazole sodium bioadhesive tablets.

关 键 词：泮托拉唑钠 生物黏附片 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]