参榆灌肠方对溃疡性结肠炎模型大鼠肠黏膜修复的影响  

The Influence of Shenyu Enema Decoction(参榆灌肠方) on Intestinal Repairment in Mice Model of Ulcerative Colitis

作　　者：朱磊[1] 沈洪[1] 王玖[2] 蒋寅[2] 司海鹏[1] 

机构地区：[1]江苏省中医院,江苏南京210029 [2]南京中医药大学,江苏南京210029

出　　处：《中医药导报》2016年第24期13-15,28,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家中医药行业科研专项资助(No.201407001);江苏省临床医学科技专项项目资助(No.BL2014100);江苏高校优势学科建设工程资助项目(江苏省中西医结合高校优势学科;PAPD);国家中医临床研究基地(脾胃)资助项目

摘　　要：目的:观察参榆灌肠方对溃疡性结肠炎模型大鼠结肠组织黏膜修复的影响。方法:采用三硝基苯磺酸(TNBS)法诱导大鼠溃疡性结肠炎模型,随机分为正常组、模型组、参榆灌肠方组和SASP组,给药10 d后处死,取结肠组织,观察结肠大体形态变化,采用HE染色法观察结肠组织病理学改变,ELISA法检测细胞因子IL-17、TGF-β水平。结果:参榆灌肠方组大鼠体质量增加优于模型组(P<0.05),结肠大体形态和病理组织学明显优于模型组;参榆灌肠方组大鼠IL-6水平明显低于模型组,TGF-水平高于模型组,差异均有统计学意义(P<0.05)。结论:参榆灌肠方灌肠可减轻溃疡性结肠炎模型大鼠肠道炎症反应,修复结肠黏膜。Objective： To observe the influence of Shenyu Enema Decoction （参榆灌肠方, SYED） on intestinal repairment in mice model of ulcerative colitis. Methods： The SD rats were randomly divided into normal group, model group, SYED group and SASP group. TNBS/ethanol was used to induce the formation of Ulcerative Colitis. Rats were treated with SYED and SASP in lO days. The serum and colonic mueosa samples of rats to observe pathological changes of colonic mucosa under light microscope was collected. Then determinate the serum IL-6 and TGF-β by ELISA. Results： The body weight of rats in SYED group increased more than that of model group （P〈0.05）. SYED group rat colon gross morphological level and pathological scores were signifi- cantly improvement, better than the model group. Compared with model group, IL-6 levels of SYED group was significantly lower and TGF-β was significantly higher （P〈0.05）. Conclusion： Enema with SYED can reduce intestinal inflammatory reaction and repair colonic mucosa in ulcerative colitis rats.

关 键 词：溃疡性结肠炎 参榆灌肠方 肠黏膜 炎症 大鼠 

分 类 号：R285.5[医药卫生—中药学]