气血宁口服液HPLC指纹图谱研究  

Study on HPLC Fingerprint of Qixuening Oral Liquid(气血宁口服液) by High Performance Liquid Chromatography

作　　者：林晓春[1] 翁立冬[2] 郑锦坤[1] 李旭桂[1] 黄升昌 陈静梅[2] 林晓佳[2] 

机构地区：[1]粤北人民医院,广东韶关512026 [2]南方医科大学,广东广州510515

出　　处：《中医药导报》2016年第24期54-57,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广东省建设中医药强省科研课题(20142143);广东医院药学研究基金(2015A32);韶关市科技计划(2014CX/K204);韶关市医药卫生计划(Y14064)

摘　　要：目的:建立气血宁口服液的高效液相指纹图谱分析方法。方法:采用HPLC法,色谱柱:Agilent HCC18(4.6 mm×250 mm,5μm);流动相:0.05%磷酸水溶液-乙腈,梯度洗脱;流速:1.0 m L/min;检测波长随时间依时间变化设定,依次为313、258、250、277、230、321、255、350 nm;柱温:25℃;进样量:10μL;检测时间90 min。结果:确定了15个共有峰,12批样品指纹图谱的相似度均在0.93以上,指认了芍药苷、阿魏酸2个特征峰,并对其余各共有峰进行了药材归属鉴定。结论:该方法准确、精密度高、重复性好,为气血宁口服液的质量控制提供了更全面的信息指标。Objective： To establish fingerprint of Qixuening Oral Liquid （气血宁口服液） by High Perfor- mance Liquid Chromatography. Methods： The separation was performed on a Agilent HC-C18（4.6 mm×250 mm,5μm） with gradient elution, with mobile phase consisting 0.05% Phosphoric acid solution and acetonitrile at the flow rate of 1.0 mL/min, column temperature 25 ℃, sample size 10 μL, test time 90 minutes. Detection wave- length, set according to different time, were 313 nm, 258 nm, 250 nm, 277 nm, 230 ran, 321 nm, 255 nm, 350 nm. Results： 15 common peaks existed in 12 samples with similarity over 0.93. Characteristic peaks of paeoniflorin, ferulic acid were recognized and common peaks were identified herb attribution. Conclusion： The method was accurate, precise, and replicable, providing more information indexes for quality control of Qixuening Oral Liquid.

关 键 词：气血宁口服液 HPLC 指纹图谱 质量控制 

分 类 号：R284.1[医药卫生—中药学]