大鼠PAH中六种血清学标志物的检测  

Analysis of six kinds of serological markers in rat PAH

作　　者：胡童谣 高戈[1] 张照奇[1] 魏丹[1] 石婷[1] 罗沙阳[2] 吴翔[3] 霍治[4] 

机构地区：[1]中南大学湘雅医学院医学检验系,湖南长沙410013 [2]中南大学湘雅医院乳腺科,湖南长沙410008 [3]海南省中医院检验科,海南海口570100 [4]中南大学基础医学院免疫学系,湖南长沙410013

出　　处：《检验医学与临床》2016年第A02期3-6,共4页Laboratory Medicine and Clinic

基　　金：湖南省自然科学基金（2015JJ4073）;中南大学硕士生自主创新项目（2016zzts501）;中南大学大学生创新创业项目（CX2015536）;湖南省教育厅科学研究项目（15C0145）;海南省卫生厅普通科研课题（14a20026）.

摘　　要：目的在血清学中寻找到合适的标志物，从而找到最适的指标应用于肺动脉高压的早期临床诊断。方法60只大鼠随机分为对照组（n=12）和模型组（n=48），实验组采用野百合碱（MCT）制备大鼠的肺动脉高压模型，对照组注射等量生理盐水。使用ELISA法测定实验性大鼠PAH模型血清中NF-KB、TNF-a、COX-2、PGE2、MMP-1、iNOS的浓度。结果各项观察指标均证明此次野百合碱大鼠模型造模成功。NF-κB、TNF-a、COX-2、PGE2、MMP-1、iNOS的浓度在第2、4、6周相比对照组均有统计学意义（P〈0．05），结合B超得到实验中的第2、4、6周均处于PAH发展过程的早期。在实验中经过对比可发现，NF-κB、TNF—α、PGE2、iNOS这4中在早期所具有的特异性最为明显。结论说明大鼠PAH模型中NF—κB、TNFa、PGE2、iNOS这4种生物标志物可反映PAH早期的病理变化，对早期PAH的研究有重要价值。Objective to find a appropriate marker for the early diagnosis of pulmonary hypertension in serum. Methods 60 rats were randomly divided into normal control group （n= 12） and experimental group （n= 48）. The experimental group was injected with MCT to make the pulmonary artery hypertension model of rats while the control group was injected with to make the same a- mount of normal saline. NF-κB,TNF-a.COX-2.PGE2.MMP-1 and iNOS levels of serum were determined by ELISA in all groups. Results the results showed that the model was successful. The concentration of NF-κB.TNF-a.COX-2.PGE2.MMP-1 .iNOS in 2,4,6 weeks had statistical significance compared to the control group（P〈0.05）. And the 2,4,6 weeks in the the experiment were in the the early stage of PAH development. We found that the specificity of NF-κB,TNF-a. PGE2.iNOS was the most ohvious in the the early. Conclusion NF-κB.TNF-u.PGE2,iNOS.in the ra＇, PAH model can reflect the early pathological changes of PAH,which has important value for the research of early PAH.

关 键 词：肺动脉高压 早期诊断 血清学标志物 

分 类 号：R512.62[医药卫生—内科学]