高浓度氢气对四氯化碳损伤性大鼠睾丸生精细胞Akt表达的影响  

The effects of high-concentration hydrogen gas on the expression of Akt in injured spermatogenic cells of rat testis induced by carbon tetrachloride

作　　者：田荆华[1] 

机构地区：[1]菏泽医学专科学校解剖学教研室,山东菏泽274000

出　　处：《右江民族医学院学报》2016年第6期574-577,共4页Journal of Youjiang Medical University for Nationalities

基　　金：山东省高等学校科技计划项目(J13LK20)

摘　　要：目的研究吸入高浓度氢气后对四氯化碳损伤性大鼠生精细胞内Akt表达的影响。方法选取健康雄性3月龄SD大鼠30只,随机平均分为对照组(Con组),四氯化碳组(CCl_4组)和氢气组(H_2组)。Con组大鼠于每周一、周五皮下注射橄榄油(0.07ml/100g),CCl_4组和H_2组于每周一、周五皮下注射30%CCl_4+70%橄榄油(0.1ml/100g),持续6周;H_2组在第6周加入高浓度H_2(3%H_2+97%空气)治疗,每天1h,连续7d。取睾丸,应用免疫组织化学和Western blot方法检测Akt的表达水平。结果免疫组化结果显示,Akt主要表达于精原细胞和初级精母细胞的胞浆中,CCl_4组大鼠生精细胞内Akt表达水平较对照组明显降低,H_2组较CCl_4组表达水平升高(P<0.01)。Western blot结果显示与免疫组化结果相一致。结论高浓度H_2可以增加CCl_4损伤性大鼠睾丸组织中Akt的表达。Objective To investigate the influence of inhalation of high-concentration hydrogen gas（H2） on the expression of Akt in injured spermatogenic cells of rats induced by carbon tetrachloride （CC14）. Meth- ods Thirty male healthy SD rats of three-month old were selected and randomly divided into 3 groups： control group （con group）, CC14 group and H2 group, each group had 10 rats. In control group, rats were subcutane- ously injected with olive oil （0. 07 ml/100g rat weight） on every Monday and Friday; in CC14 group and H2 group,rats were subcutaneously injected with 30% carbon tetrachloride plus 70% olive oily solution （0.1 ml / 100 g rat weight） on every Monday and Friday for 6 weeks. Rats of H2 group were added with inhalation of high-concentration hydrogen gas （3% H2 ＋ 97% air）, an hour everyday for 7 days since the sixth week. Testes were taken out for detecting the Akt expresstion levels by using immunohistoehemistry and Western blot. Results Immunohistochemical staining results showed that Akt mainly expressed in cytoplasm of spermatogoni- um and primary spermatocyte. Compared with control group, the expression of Akt in spermatogenic cells of rats was significantly decreased in CC14 group l compared with CC14 group, the expression of Akt was signifi- cantly increased in H2 group （ P 〈0.01）. The Western blot test results were consistent with the immunohistochemical test results. Conclusion High-concentration hydrogen gas can increase the expression of Akt in rat testicular tissues induced by carbon tetrachloride injury.

关 键 词：氢气 四氯化碳 生精细胞 大鼠 AKT 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]