甘油稀释液的加入方法对公牛精液冷冻后活力的影响  

The Effects of Different Ddding Method of Glycerin Diluents on Frozen Bull Sperm Motility

作　　者：金穗华[1] 王鹏武 胡树香 王振云[1] 马国辉 丁瑞 

机构地区：[1]南京农业大学,江苏南京210095 [2]云南省大理白族自治州家畜繁育指导站 [3]内蒙古赛科星繁育生物技术股份有限公司

出　　处：《畜牧兽医杂志》2017年第1期21-23,共3页Journal of Animal Science and Veterinary Medicine

基　　金：云南省科技厅生物重大专项(奶业专项)项目(2014ZA007)

摘　　要：为探讨甘油稀释液的加入方法对公牛精液冷冻后精子活力的影响,以不同的加入方法(A组一次加入法,B组二次加入法)进行了试验比较。结果表明:稀释、平衡后平均精子活力A组低于B组(P<0.05);精清中谷草转氨酶(AST)和乳酸脱氢酶(LDH)平均渗出量A组高于B组(P<0.05)。冷冻后平均精子活力A组低于B组(P<0.05);精清中谷草转氨酶(AST)和乳酸脱氢酶(LDH)平均渗出A组高于B组(P<0.05)。甘油稀释液采用二次加入法优于一次加入法。To study the effects of different adding methods of glycerin diluents on the frozen bull sperm motility, two meth- ods were experimented （A group was added once, B group was twice）. The results showed that after the treatment of being diluted and balanced of semen, the motility of A group was lower than B （P〈0.05）, the average seepage quantity of aspartate aminotransferase （AST） and lactate dehydrogenase （LDH） in seminal plasma, A group was higher than B （P〈0.05）. After frozen, the average motility of A group was lower than B （P〈0.05）, the average seepage quantity of aspartate aminotransferase （AST） and lactate dehydrogenase （LDH） in seminal plasma, A group was higher than B （P〈0.05） , The method of adding the diluents to glycerin in twice is better than the once.

关 键 词：公牛精液 甘油稀释液 加入方法 冷冻 精子活力 

分 类 号：S814.4[农业科学—畜牧学]