Cu-MOF催化过氧化氢应用于多巴胺的检测  

Detection of Dopamine based on Catalytic Activity of Cu-MOF towards H_2O_2

作　　者：苗向阳[1] 郁惠珍[1] 王杨[1] 顾准[1] 王兆寅[2] 戴志晖[2] 

机构地区：[1]苏州健雄职业技术学院生物与化学工程系,太仓215411 [2]江苏省生物功能材料重点实验室南京师范大学化学与材料科学学院,南京210023

出　　处：《化学通报》2017年第1期47-52,共6页Chemistry

基　　金：国家自然科学基金项目(21175069)资助

摘　　要：本文利用一种具有H_2O_2催化活性的Cu-MOF[Cu_3(BTC)_2(H_2O)_3,简称HKUST-1],构建了以邻苯二胺(OPD)为颜色指示分子的比色传感体系,实现了对H_2O_2和多巴胺(DA)的快速灵敏检测。HKUST-1起到催化H_2O_2氧化OPD的作用,反应体系能够呈现出显著的颜色变化。在优化条件下,415nm处的吸收峰强度与H_2O_2浓度呈双线性关系,线性范围分别为10~50 mmol/L和50~100 mmol/L,相对标准偏差分别为0.9947和0.9995,最低检出限为1.29mmol/L。由于DA能抑制H_2O_2氧化OPD,因此比色传感体系还可以用于快速检测DA,线性范围分别为0.25~5μmol/L和2.5~25μmol/L,相对标准偏差分别为0.9783和0.9705,最低检出限为0.262μmol/L。该项工作拓展了Cu-MOFs材料在生物分子催化和生物传感方面的应用。In this work,a colorimetric biosensing platform for H_2O_2 and dopamine（DA） was constructed based on the catalytic activity of one type of Cu-MOF [Cu_3（BTC）_2（H_2O）_3,HKUST-1]. The color of o-phenylenediamine（OPD） can be changed significantly because of the oxidation of H_2O_2 catalyzed by HKUST-1. As a result,H_2O_2 and DA were detected rapidly and sensitively by our system. Under the optimum conditions,two linear relationships between the intensity of absorption peak at 415 nm and the concentration of H_2O_2（from 10 to 50 mmol/L and from 50 to 100 mmol/L,R = 0. 9947 and 0. 9995） were obtained with the detection limit of 1. 29 mmol/L. Besides,DA can resist the oxidation of OPD from H_2O_2,this platform was further used for rapid detection of DA with two linear ranges（from 0. 25 to 5μmol/L and from 2. 5 to 25μmol/L,R = 0. 9783 and 0. 9705）,and the detection limit was 0. 262 μmol/L.Therefore,this work may broaden the applications of Cu-MOFs in biological molecular catalysis and biosensing fields.

关 键 词：Cu-BTC 比色分析 过氧化氢 多巴胺 多相催化剂 

分 类 号：O627[理学—有机化学] O657.3[理学—化学]