醒脑益智方对T2DM痴呆小鼠性激素信号通路的影响  

Effect of Xingnao Yizhi Prescription on Sex Hormone Signaling in Diabetic Dementia Mice

作　　者：高晓斐[1] 徐萌[1] 张静[1] 岳仁宋[2] 

机构地区：[1]成都中医药大学,成都610072 [2]成都中医药大学附属医院,成都610072

出　　处：《中国实验方剂学杂志》2017年第2期81-85,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81072775);四川省学术和技术带头人培养资金项目(CRS2014001);四川省科技计划项目(2015SZ0100)

摘　　要：目的:探讨醒脑益智方对2型糖尿病痴呆小鼠血清性激素水平及雌激素受体(estrogen receptor,Er)α,ErβmRNA表达的影响及其作用机制。方法:设昆明小鼠为空白组,将KK-Ay小鼠血糖≥13.9 mmol·L-1者随机分为模型组、吡拉西坦组、醒脑益智方组。吡拉西坦组ig吡拉西坦片(150 mg·kg-1),醒脑益智方组ig醒脑益智方(7.2 g·kg-1),空白组和模型组每天用等体积生理盐水ig。给药前后行Morris水迷宫测试并记录潜伏期。小鼠麻醉后迅速取血、大脑海马及额叶皮质。酶联免疫吸附法(ELISA)测定血浆性激素水平,包括卵泡刺激素(FSH),孕酮(P),雌二醇(E2),睾酮(T)等;实时荧光定量PCR检测Erα,ErβmRNA表达水平。结果:与模型组比较,空白组、吡拉西坦组和醒脑益智方组第5天潜伏期明显减少(P<0.05);与模型组比较,空白组、醒脑益智方组促黄体生成素(LH),T水平明显下降(P<0.05),E2,P水平明显升高(P<0.05);与吡拉西坦组比较,醒脑益智方组LH水平明显下降(P<0.05)。与模型组比较,吡拉西坦组、醒脑益智方组Erβ受体mRNA表达水平明显增高(P<0.05),其他各组间差异均不明显。结论:糖尿病痴呆的出现可能与性激素通路有关,醒脑益智方对糖尿病轻度认知功能障碍有明显改善作用,这一作用可能通过提高血浆E2,P水平及雌激素受体Eβ表达水平,降低LH,T水平实现。Objective： To investigate the effect and mechanism of Xingnao Yizhi prescription on mRNA expression levels of serum hormone and estrogen receptor（ Erα and Erβ） for type 2 diabetic dementia mice.Method： Kunming mice were set as blank group. KK-AY mice with blood sugar ≥13. 9 mmol·L^- 1were randomly divided into model group, piracetam group, and Xingnao Yizhi prescription group. Piracetam tablets（ 150mg·kg^- 1） were given by ig administration in piracetam group. Xingnao Yizhi prescription（ 7. 2 g·kg^- 1） was given by ig administration in Xingnao Yizhi prescription group. Equal volume of saline was given in normal group and model group by ig administration. Morris water maze test was conducted before and after drug administration,and latent period was recorded. Plasma,hippocampus samples and frontal cortex were collected as soon as possible after anesthesia in mice. Enzyme linked immunosorbent assay（ ELISA） was used to test plasma sex hormone level,such as follicle-stimulating hormone（ FSH）,progesterone（ P）,estradiol（ E2）,and testosterone（ T）. Real-time PCR was used to test Erα,Erβ mRNA expression levels. Result： As compared with model group,the latent period was significantly shortened in blank group,piracetam group,and Xingnao Yizhi prescription group（ P〈0. 05）; LH and T levels were significantly reduced in blank group and Xingnao Yizhi prescription group（ P〈0. 05）,and E2 and P levels were significantly increased（ P〈0. 05）. As compared with piracetam group,LH level was significantly reduced in Xingnao Yizhi prescription group（ P〈0. 05）. As compared with the model group,Erβ mRNA expression level was significantly increased in piracetam group and Xingnao Yizhi prescription group（ P〈0. 05）. Conclusion：The emergence of diabetic dementia may be related to the sex hormone pathway. Xingnao Yizhi prescription can improve the mild cognitive impairment in patients with diabetes mellitus,and the mechanism may be associated with improving plasma E

关 键 词：醒脑益智方 糖尿病痴呆 血浆性激素水平 雌激素受体Α 雌激素受体Β 

分 类 号：R285.5[医药卫生—中药学]