白藜芦醇对妊娠期糖尿病大鼠肾脏组织抗氧化和抗炎的作用  

Anti-oxidation and Anti-inflammatory Effects of Resveratrol on Renal Tissue in Gestational Diabetic Rats

作　　者：张雪 邢宝恒[2] 曹亚磊[2] 董秀娟[2] 李芹[2] 

机构地区：[1]孟村回族自治县医院,河北沧州061400 [2]沧州市中心医院,河北沧州061001

出　　处：《中国实验方剂学杂志》2017年第2期131-137,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：沧州市科技支撑计划项目(151302166)

摘　　要：目的:本实验以妊娠期糖尿病大鼠为研究对象,探讨白藜芦醇对妊娠期糖尿病大鼠肾脏组织抗氧化和抗炎的作用。方法:实验用雌性大鼠经高脂高糖饲料喂养8周后与雄鼠合笼过夜制备孕鼠,5 d后采用ip链脲佐菌素(STZ,35 mg·kg^(-1))破坏胰岛β细胞的方法诱导制备糖尿病大鼠,选取80只模型大鼠根据血糖水平随机分为模型组、白藜芦醇60,120,240 mg·kg^(-1)组治疗组和盐酸二甲双胍(200 mg·kg^(-1))阳性药组,并另取16只同批妊娠5 d大鼠作为正常妊娠组,以及16只同龄非妊娠雌性大鼠作为正常非妊娠组;判定造模成功后立即ig给药,每天1次,疗程2周。给药治疗前和治疗完成后分别观察各组大鼠一般状况;治疗完成后测定各组大鼠空腹血糖水平,测量24 h尿量并测定24 h尿蛋白量(UPro),检测血清中血尿素氮(BUN),肌酐(SCr),尿酸(UA)含量,通过PAS染色观察肾脏组织病变,通过原位末端转移酶标记法(TUNEL)染色观察肾脏组织细胞凋亡状况并计算凋亡指数;测定血清中总抗氧化能力(T-AOC)水平和肾脏组织中超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px),过氧化氢酶(CAT)活性以及丙二醛(MDA)含量,检测血浆中炎症因子C-反应蛋白(CRP),肿瘤坏死因子-α(TNF-α),白细胞介素-1β(IL-1β),白细胞介素-6(IL-6),细胞间黏附分子-1(ICAM-1)水平。结果:正常妊娠组大鼠各监测指标与正常非妊娠组比较均无显著性差异;与正常妊娠组比较,模型组大鼠"三多一少"症状明显,血糖水平显著升高,24 h尿量显著增多,UPro显著增高,血清中BUN,SCr,UA含量显著升高,呈现明显的肾脏组织病变和肾细胞凋亡、凋亡指数显著升高,血清中T-AOC水平和肾脏组织中SOD,GSH-Px,CAT活性均显著降低,MDA含量显著升高,上述差异均具有统计学意义(P<0.05,P<0.01);与模型组比较,白藜芦醇治疗组大鼠一般状况明显改善,"三多一少"症状明显减轻;白藜芦醇120,240 mg·kg-1治疗组Objective： To investigate the anti-oxidation and anti-inflammatory effects of resveratrol（ Res） on renal tissues in gestational diabetic rats. Method： Female rats were fed with high fat and high sugar diet for 8 weeks and then kept in the same cage with male rats to prepare pregnant rats. After 5 days,the pregnant rats were injected with STZ（ 35 mg·kg^-1） by intraperitonal to make gestational diabetic models. 80 model rats were selected andrandomly devided into model group, Res（ 60, 120, 240 mg ·kg^-1） groups and Metformin hydrochloride（ 200 mg·kg^-1） group. Another 16 gestational rats in the same batch were selected as normal gestational group,and 16 non-gestational female rats at the same age were selected as normal non-gestational group. After successful modeling,the medicines were given by intragastric administration for 2 weeks,once a day.Before and after treatment,the general status of rats in every group were observed. After treatment,the levels of blood sugar were determined; the level of 24 h urine protein（ UPro） was determined; the levels of blood urea nitrogen（ BUN）, creatinine（ SCr）, and uric acid（ UA） in serum were determined; the renal tissue histopathological changes were observed by periodic acid-schiff staining（ PAS） method; the renal cells apoptosis was observed by terminal-deoxynucleoitidyl transferase mediated nick end labeling（ TUNEL） staining method,and the apoptosis index was alculated; the levels of total antioxidative capacity（ T-AOC） in serum and the activities of superoxide dismutase（ SOD）,glutathione peroxidase（ GSH-Px）,catalase（ CAT） as well as the content of malondialdehyde（ MDA） in renal tissues were determined; the levels of C-reactionprotein（ CRP）,tumor nerosis factor-α（ TNF-α）,interlukin-1β（ IL-1β）,interlukin-6（ IL-6）,and intercellular cell adhesion molecule-1（ ICAM-1） in plasma were also determined. Result： There were no differences in various monitoring indexes between the normal gestat

关 键 词：白藜芦醇 妊娠期糖尿病 肾脏 抗氧化 抗炎 

分 类 号：R285.5[医药卫生—中药学]