鸭源鸡杆菌外膜蛋白W基因克隆及结构与功能分析  

Cloning of the outer membrane protein W gene of Gallibacterium anatis and analysis of its structure and function

作　　者：敬文宪 彭志锋[1] 王新卫[1] 常洪涛[1] 刘红英[1] 王川庆[1] 卢彩景 杨霞[1] 

机构地区：[1]河南农业大学禽病研究所,河南郑州450002

出　　处：《中国兽医学报》2017年第1期47-53,共7页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金项目资助(31302090);河南省高校科技创新团队与支持计划资助项目(14IRTSHN015)

摘　　要：参考GenBank中鸭源鸡杆菌(Gallibacterium anatis,G.anatis)UMN179的外膜蛋白W(outer membrane protein W,OmpW)基因序列设计1对引物,对鸭源鸡杆菌PDS-RZ-1-SLG株的OmpW基因进行克隆、测序,并通过生物信息学软件对该蛋白结构与功能进行分析及预测。结果显示:OmpW基因大小为705bp,编码234个氨基酸;与鸭源鸡杆菌UMN179株、F149株及12656/12株的OmpW氨基酸同源性分别为88.9%、78.7%和79.6%;OmpW相对分子质量为25 300,等电点为7.88,是能够稳定存在的蛋白,N端有1个疏水性的α螺旋信号肽,C端有1个疏水性的β折叠区域,并且在外膜表面存在1个保守性的B细胞线性表位。本试验成功克隆了鸭源鸡杆菌PDS-RZ-1-SLG株的OmpW基因,并对其结构与功能进行初步分析和预测,为进一步研究其生物学功能及其应用奠定基础。A pair of primers was designed according to G. anatis UMN179 OmpW gene sequence published in the GenBank, and the OmpW gene of G. anatis PDS-RZ-1-SLG was cloned and se- quenced. In addition,its structure and function of OmpW were analyzed and predicted by a series of bioinformatics software. The results showed that the OmpW gene was 705 bp in size,encoding 234 amino acid residues. Compared with the amino acid sequences of the OmpW1 of G. anatis UMN179,F149 and 12656/12, the homology were 88. 9%,78. 7% and 79. 6%,respectively. OmpW is a stable protein, with a relative molecular weight of 25 300 and a isoelectric point of 7.88,containing a hydrophobic leader peptide region,which is likely to consist of a long stretch of helical structures in N-terminal end, and a hydrophobic region with fl-structure in C-terminal end. And there is a conservative B cell linear epitope antigen on the surface of the outer membrane which has a potential to elicit cross-protective immunity. In this study, the G. anatis PDS-RZ-1- SLG OmpW gene was cloned and the structure and function of OmpW were analyzed and predicted pre- liminarily. The results laid the foundation for the further studies on the function and application.

关 键 词：鸭源鸡杆菌 OmpW基因 克隆 结构与功能分析 

分 类 号：S852.61[农业科学—基础兽医学]