IDO在梅花鹿茸角中的表达及对鹿茸软骨细胞分化的影响  

Expression of IDO in sika deer antler and its effects on antler chondrocyte differentiation

作　　者：耿爽[1] 杨占清[1] 张虹亮 成文革[2] 于海帆 郭斌[1] 岳占碰[1] 

机构地区：[1]吉林大学动物医学学院,吉林长春130062 [2]吉林省生物研究所,吉林长春130012

出　　处：《中国兽医学报》2017年第1期118-122,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31472158;31372390)

摘　　要：以梅花鹿茸角为研究对象,通过原位杂交方法检测吲哚胺2,3-双加氧酶1(IDO1)和吲哚胺2,3-双加氧酶2(IDO2)在茸角中的表达。在培养的鹿茸软骨细胞中分别添加IDO1抑制剂1-甲基-L-色氨酸(1-L-MT)和IDO2抑制剂1-甲基-D-色氨酸(1-D-MT),作用24h后,通过荧光定量PCR方法检测软骨细胞分化标志分子Ⅹ型胶原(ColⅩ)表达的变化,进一步研究IDO1和IDO2对鹿茸软骨细胞分化的影响。结果显示:IDO1和IDO2主要在鹿茸真皮层成纤维细胞、间充质细胞和软骨细胞中表达。用浓度分别为200μmol/L和100μmol/L的1-L-MT和1-DMT处理鹿茸软骨细胞24h后,荧光定量PCR结果显示,ColⅩmRNA在鹿茸软骨细胞中的表达量均显著下降。结果表明:当IDO的活性受到抑制时,鹿茸软骨细胞的分化过程也受到明显抑制,IDO可能在梅花鹿鹿茸软骨细胞分化过程中起重要的作用。This experiment aimed at a study on the expression of indoleamine 2,3-dioxygenase 1 （IDOl） and indoleamine 2,3-dioxygenase 2 （IDO2） in deer antler by in situ hybridization, and further detect the influence of IDO on the differentiation of antler chondrocytes. The antler chon- drocytes were treated with IDOl inhibitor 1-L-MT and IDO2 inhibitor 1-D-MT for 24 h,and ana- lyzed the expression of chondrocyte differentiation marker collagen X （Col X ） by real-time PCR. The results showed that both the IDOl and IDO2 were mainly localized in the dermal fibroblasts, mesenchyme cells and chondrocytes in the antler. By real-time PCR analysis,200 μmol/L 1-L-MT and 100 μmol/L 1-D-MT could inhibit the expression of Col X mRNA in the antler ehondrocytes at 24 h. These results demonstrate that inhibition of IDO activity could block the differentiation of antler chondrocytes and IDO may play an important role in the process of chondroeyte differentia- tion in sika deer antler.

关 键 词：吲哚胺2  3-双加氧酶 鹿茸 软骨细胞 分化 梅花鹿 

分 类 号：S852[农业科学—基础兽医学]