迷走神经电刺激对脑外伤后昏迷大鼠前额叶皮质去甲肾上腺素α1受体表达变化的影响  

Effect of vagus nerve stimulation on expression of α1-adrenergic receptor in prefrontal cortex of coma rats induced by traumatic brain injury

作　　者：陈琴[1] 黄菲菲[1] 董晓阳[1] 冯珍[1] 

机构地区：[1]南昌大学第一附属医院康复医学科,南昌330006

出　　处：《中国康复医学杂志》2017年第1期28-32,共5页Chinese Journal of Rehabilitation Medicine

基　　金：国家自然科学基金资助项目(81260295)

摘　　要：目的:探讨迷走神经电刺激(vagus nerve stimulation,VNS)对脑外伤(traumatic brain injury,TBI)后昏迷大鼠的促醒作用及其可能机制。方法:健康成年SD大鼠90只,随机分为空白对照组、假刺激组和刺激组。TBI后昏迷大鼠给予VNS治疗,观察其行为学变化,并通过免疫组织化学和Western-blot技术检测各实验组大鼠前额叶皮质(prefrontal cortex,PFC)组织中去甲肾上腺素α1受体(α1R)含量。结果:假刺激组和刺激组大鼠各30只,观察行为学变化发现,刺激组中20只出现翻正反射,假刺激组仅8只出现翻正反射;将3个实验组中α1R含量进行两两比较,发现刺激组α1R水平均显著高于其他两组(P<0.05)。结论:VNS可改善TBI后昏迷大鼠的意识状态水平,对促进TBI后昏迷大鼠的觉醒有积极的治疗作用,其机制可能与PFC去甲肾上腺素α1R水平上调有关。Objective： To investigate the wake-promoting action of vagus nerve stimulation（VNS） in traumatic brain injury（TBI） induced coma rats and its possible mechanism.Method： Ninety healthy Sprague Dawley（SD） rats were randomly divided into 3 groups： control, sham-stimulated（TBI） and stimulated（TBI＋VNS） group. VNS was applied to rats after TBI induced coma models. Then,to observe the changes in the behavior of rats and to detect the content of α1-adrenergic receptor in the prefrontal cortex（PFC） by using immunohistochemistry and Western-blotting techniques.Result： Twenty rats in the stimulated group exhibited righting reflex and 8 rats had the same responses in TBI group. In stimulated group, the level of α1-adrenergic receptor in PFC were significantly higher than those in other groups（P〈0.05）.Conclusion： VNS can improve the level of consciousness of the rats after TBI-coma, and has a positive effect on wake-promoting action. The potential mechanism might be related to upregulating the expression of α1-adrenergic receptor in PFC.

关 键 词：迷走神经电刺激 脑外伤 昏迷 促醒 去甲肾上腺素α1受体 

分 类 号：R651.15[医药卫生—外科学]