间歇性低氧与规律运动对中老龄果蝇心脏泵血能力及生活质量的影响  

Effects of Intermittent Hypoxia and Regular Exercises on Myocardial Systolic Function and Life Quality of Middling Aging Drosophilae

作　　者：王卉[1] 郑澜[1] 文登台 奉悦 倪柳[1] 张民[1] 

机构地区：[1]湖南师范大学体适能与运动康复湖南省重点实验室,湖南长沙410012

出　　处：《中国运动医学杂志》2017年第1期26-35,共10页Chinese Journal of Sports Medicine

基　　金：教育部博士点基金资助项目(20134306110009)

摘　　要：目的:探讨适宜的规律运动与间歇性低氧对中老龄果蝇心脏泵血功能与生活质量的影响。方法:通过扩大培养收集8 h内羽化的W1118品系处女蝇4500只,随机分为三个低氧年龄段(n=1500):青年低氧组(1~2周)、中年低氧组(3~4周)与老年低氧组(5~6周)。低氧结束后利用心力衰竭模型筛选出最佳低氧年龄段与低氧持续时间。再通过扩大培养收集8 h内羽化的W1118品系处女蝇1200只,随机分为常氧安静组(NC组)、常氧运动组(NE组)、低氧安静组(HC组)和低氧运动组(HE组)。HC与HE组果蝇采用6%O2与94%N2的混合气体,每天低氧6 h,NE与HE组果蝇每天运动2.5 h,低氧与运动干预结束的第二天进行心脏泵血能力与生活质量相关指标的检测。结果:(1)果蝇的心力衰竭率随年龄的增长而上升,中年组及老年组果蝇心力衰竭率随低氧暴露时间的延长逐渐下降。(2)NE组果蝇心脏舒张直径显著高于NC组与HC组(P<0.05,P<0.01),HC组显著低于NC组与HE组(P<0.05,P<0.01);HC组果蝇收缩直径显著低于NC组与HE组(P<0.05,P<0.01);NC组果蝇心脏射血分数显著低于NE组(P<0.01)。(3)NE组、HC组与HE组果蝇攀爬速度均显著高于NC组(P<0.05,P<0.01)。(4)HC组及HE组果蝇夜晚睡眠总时间显著长于NC组(P<0.05,P<0.01),HC组果蝇夜晚睡眠段数显著小于NC组(P<0.01)。(5)NE组果蝇活动总量显著高于其他三组(P<0.05),而HC组显著低于NC组(P<0.05),NE组果蝇清醒睡眠时间比以及单位活动时活动量均显著高于其他三组(P<0.05),HC组果蝇单位活动时活动量显著高于NC组与HE组果蝇(P<0.05),其日间活动时间显著低于NC组。(6)HC组果蝇最高寿命显著高于NC组与NE组(P<0.01)。HC组果蝇平均寿命显著高于其他三组(P<0.01),HE组果蝇平均寿命显著高于NC组与NE组(P<0.05)。结论:规律运动与间歇性低氧均能增强中老龄果蝇的攀爬能力与日常活动能力,但与规律运动能增强中老龄果蝇心脏泵血能力不同,间歇�Objective To study the effects of intermittent hypoxia and regular exercise on myocardial pump function and life quality of middling aging drosophilae. Methods Totally 4,500 virgin W1118 fe- male flies were collected and divided into three groups by age： young-hypoxia group（YH）receiving hy-poxia of one to two weeks,middle-age-hypoxia group（MH）undergoing hypoxia of 3 to 4 weeks and old- hypoxia group（OH）receiving hypoxia of 5 to 6 weeks,each of 1500. The best hypoxic age group and duration were selected after hypoxia treatment. Then 1,200 virgin W1118 female flies were collected and divided into a normoxic control （NC） group, a normoxic exercise （NE） group, a hypoxic control （HC） group and a hypoxic exercise（HE）group,each of 400. The HC and HE groups stayed in environment with ox- ygen content of 6% for 6 h per day, and the daily exercise of NE and HE groups were 2.5 h. The myocardial pump function and life quality were evaluated for all groups after the intervention. Results （1）The heart failure rate increased with age,and that of MH and OH groups decreased with the extension of hypoxic duration.（2）The diastolic diameter of group NE was significantly higher than group NC and HC （P〈0.05, P〈0.01）, and that of group HC was significantly lower than group NC and HE （P〈 0.05）. The systolic diameter of group HC was significantly lower than group NC and HE（P〈0.05）. The cardiac ejection fraction of group NE was significantly higher than group NC（P〈0.01）.（3）The average climbing speed of group NE, HC and HE were all significantly faster than group NC （P〈0.05,P〈 0.01）.（4）The total sleep duration at night of group NC was significantly shorter than group HC and HE （P〈0.05） ,but the number of sleep bouts at night of the former was significantly more than group HC （P〈0.01）. （5）The average total activity of group NE was significantly higher than the other 3 groups （P〈0.05） ,and that of group HC was significantly lower than group

关 键 词：间歇性低氧 规律运动 心力衰竭模型 心脏泵血能力 生活质量 

分 类 号：R87[医药卫生—运动医学]