草石蚕外植体消毒方法及不定芽增殖培养基研究  

Study on Explant Disinfection Method of Stachys siebordii Miq. and Adventitious Bud Proliferation Medium

作　　者：孟祥玉[1] 张素勤[1] 耿广东[1] 熊娅[1] 

机构地区：[1]贵州大学农学院,贵阳550025

出　　处：《湖北农业科学》2016年第24期6601-6604,共4页Hubei Agricultural Sciences

基　　金：科技部;财政部"富民强县"专项行动计划项目(205)

摘　　要：以草石蚕茎尖和叶片为外植体,研究了草石蚕（Stachys sieboldii Miq.）无菌系建立的方法和不定芽增殖适宜的培养基配方,为草石蚕组织培养快速繁殖和脱病苗培育奠定基础。结果表明,NaClO溶液比HgCl_2溶液消毒效果好;茎尖和叶片在NaClO溶液中适宜的消毒时间分别为10~15 min和15 min,其污染率均为0,并且外植体未发生褐化现象。不定芽增殖的最佳培养基为MS＋6-BA 5.0 mg/L＋NAA 0.3 mg/L＋GA31.5 mg/L,每个外植体可诱导6.6个不定芽,并且生长健壮。The buds and leaves were applied as explants in order to investigate suitable sterilizing method and proliferation medium in Stachys siebordii Miq. in the present study. The results showed that the sterilizing effect of NaClO solution was better than that of HgCl_2 solution,and contamination percentage was 0,and no browning happened when the buds and leaves explants were sterilized in 1% NaClO solution for 10~15 min and 15 min respectively. The best medium for adventitious bud proliferation was MS＋6-BA 5.0 mg/L＋NAA 0.3 mg/L＋GA31.5 mg/L,and 6.6 strong adventitious buds per explant were produced on the medium.

关 键 词：草石蚕(Stachys sieboldii Miq) 外植体 消毒 不定芽增殖 

分 类 号：Q943.1[生物学—植物学]