RP-HPLC法同时测定山茱萸中莫诺苷和马钱苷的含量  

Determination of the Morroniside and Loganin in Cornus officinalis by HPLC

作　　者：李佳[1] 蒲晓辉[1] 宗兰兰[1] 袁琦[1] 杨浩[1] 

机构地区：[1]河南大学药物研究所,河南开封475004

出　　处：《河南大学学报（医学版）》2016年第4期249-251,共3页Journal of Henan University:Medical Science

基　　金：NSFC-河南人才培养联合基金(U1304826)

摘　　要：〔目的〕建立同时测定山茱萸中莫诺苷和马钱苷含量的方法。〔方法〕采用反相HPLC法和依利特ODS-BP（250mm×4.6 mm）色谱柱,以甲醇-水为流动相梯度洗脱,流速1.0 mL/min,检测波长为237 nm。〔结果〕莫诺苷和马钱苷质量浓度分别在0.099 0-0.4950 mg/mL、0.0400-0.2400 mg/mL范围内具有良好的线性关系,相关系数分别是0.9999、0.9998,平均回收率分别为97.4%（RSD=1.68%,n=9）、98.6%（RSD=1.30%,n=9）。〔结论〕HPLC法测定山茱萸中莫诺苷和马钱苷的含量,线性关系良好、准确度高、稳定性好、重复性好,可作为该药品的质量控制方法。〔O bjective〕To establish a method for the determination of morroniside and loganin in Cornus officinalis.〔Methods〕The sample was analyzed on a Hypersil-ODS chromatographic column（ 250 mm × 4. 6 mm, 5 μm）, with a gradient elution of methanol-water at the flow rate of 1. 0 mL/min, and the detection wavelength was set at 237 nm. 〔R esults〕The calibration curves were linear within the range of 0. 099 0 - 0. 495 mg/mL for morroniside, and 0. 040 0 - 0. 240 mg/mL for loganin, respectively. The average recoveries of morroniside and loganin acid were 97. 4% and 98. 6%,and RSDs were 1. 68% and 1. 30%,respectively.〔Conclusion〕It showed that the HPLC method for determination of morroniside and loganin in Cornus officinalis has the following advantages： good linearity, high accuracy, good stability and good reproducibility. It was very significant for the quality control of the drug.

关 键 词：HPLC法 山茱萸 莫诺苷 马钱苷 含量测定 

分 类 号：R927.2[医药卫生—药学]