钒接触与热休克蛋白70-hom基因多态性对工人氧化应激影响  

Impact of occupational vanadium exposure and HSP70-hom polymorphism on workers' oxidative stress level

作　　者：吴玲玉[1] 刘云兴 崔鹂[3] 李顺品[2] 张祖辉[4] 兰亚佳[1] 张勤[1] 

机构地区：[1]四川大学华西公共卫生学院环境卫生与职业医学系,四川成都610041 [2]攀枝花市第二人民医院职业病科,攀枝花617068 [3]深圳市龙岗区疾病预防控制中心职业病防治科,广东深圳518100 [4]四川大学华西基础医学与法医学院生物化学与分子生物学教研室,610041

出　　处：《中国职业医学》2016年第6期666-672,共7页China Occupational Medicine

基　　金：攀枝花市主要职业病防治相关技术服务及分子遗传标志物检测分析项目(11H1219)

摘　　要：目的探讨职业钒接触和热休克蛋白70（HSP70）-hom基因多态性对工人氧化应激水平影响的交互作用。方法采用判断抽样方法,以从事钒接触作业的87名工人为钒接触组,63名化工厂工人为对照组;检测血清总超氧化物歧化酶（SOD）、锰SOD、铜锌SOD、诱导型一氧化氮合酶（i NOS）的活力和MDA水平,采用限制性片段长度多态性-聚合酶链反应法检测HSP70-hom基因型分布,分析HSP70-hom基因多态性对钒接触人群氧化应激水平的综合影响。结果与对照组比较,钒接触组人群血清总SOD和锰SOD活力均下降（P〈0.01）,MDA水平升高（P〈0.01）。HSP70-hom的T/T基因型组人群血清总SOD活力低于T/C＋C/C基因型组人群（P〈0.01）。单因素Logistic回归分析结果显示,HSP70-hom基因型与钒接触的交互项与血清总SOD活力和MDA高水平的发生均有关联（P〈0.05）。多因素Logistic回归分析和叉生分析结果显示,HSP70-hom基因型与钒接触对血清总SOD活力偏高发生的具有相乘交互作用[比值比（OR）及其95%可信区间（CI）为6.051（1.001~36.572）,P=0.05],并具有相加的交互作用,交互作用归因比及其95%CI为0.935（0.086~1.783）;血清MDA水平偏高的发生仅与钒接触有关[OR（95%CI）为6.352（3.099~13.021）,P〈0.01],钒接触与HSP70-hom基因型对血清MDA水平偏高的发生无交互作用。未发现钒接触或HSP70-hom基因多态性对血清铜锌SOD和i NOS活力有影响。结论钒及其化合物可通过影响血清SOD和MDA而导致钒接触人群发生氧化损伤;钒接触与HSP70-hom基因多态性对作业工人血清总SOD活力偏高的发生具有交互作用。Objective To investigate the interaction of occupational vanadium exposure and heat shock protein 70-hom（ HSP70-hom） gene polymorphism on the oxidative stress level of workers. Methods By judgment sampling,87 workers from vanadium products plant as vanadium exposure group and 63 workers from chemical industry as control group were enrolled into this investigation. The activities of total superoxide dismutase（ T-SOD）,manganese-containing superoxide dismutase（ Mn-SOD）,copper and zinc-containing superoxide dismutase（ Cu Zn-SOD） and inducible nitric oxide synthase（ iNOS）,and the level of malondialdehyde（ MDA） in the serum of studied subjects were detected. The HSP70-hom genotype was examined by restricted fragment length polymorphism-polymerase chain reaction. Based on the above data,the comprehensive impact of HSP70-hom gene polymorphism and vanadium exposure on workers＇ oxidative stress level was analyzed. Results Compared with the control one,the activities of serum T-SOD and Mn-SOD of vanadium-exposed group were significantly decreased（ P〈0. 01）,while the level of serum MDA was significantly increased（ P〈0. 01）.Furthermore,the serum T-SOD activity of T / T genotype was statistically lower than that of T / C ＋ C / C genotype（ P〈0. 01）. Both high serum（ T-SOD） activity and high MDA level were significantly influenced by the interaction between vanadium exposure and HSP70-hom polymorphism by univariate Logistic regression analysis（ P〈0. 05）. Using multiple Logistic regression and crossover analysis,multiplicative and additive interactions on high serum（ T-SOD） activity between vanadium exposure and HSP70-hom polymorphism was observed [odds ratio（ OR） was 6. 051,95% confidence interval（ CI） ： 1. 001-36. 572,P = 0. 05]and the attributable proportion due to interaction was 0. 935（ 95% CI： 0. 086-1. 783）.However,high serum MDA level was significantly associated with vanadium exposure [OR（ 95% CI） ： 6. 352（ 3. 099-13. 021）,P〈0. 01] wi

关 键 词：钒 热休克蛋白70 基因多态性 氧化应激 

分 类 号：R135[医药卫生—劳动卫生] R114[医药卫生—公共卫生与预防医学]