苯并(a)芘对HELF细胞S期细胞周期相关蛋白的作用  

Effect on the cell cycle proteins of arrested S phase cells in human embryo lung fibroblasts cells induced by benzo(a) pyrene

作　　者：祁妍敏[1] 姚碧云[2] 于红燕[1] 赵鹏[2] 周宗灿[2] 

机构地区：[1]中国民用航空局民用航空医学中心,北京100123 [2]北京大学医学部公共卫生学院毒理学系

出　　处：《毒理学杂志》2016年第6期418-421,共4页Journal of Toxicology

基　　金：民航局科技项目(MHRD201042)

摘　　要：目的观察由于苯并(a)芘(Ba P)作用阻滞于S期的人胚肺成纤维细胞(HELF)的细胞周期蛋白和关卡蛋白表达水平的改变,探讨Ba P影响细胞周期的作用机制。方法 5μmol/L Ba P处理4 h,于处理后24 h收获细胞,利用流式细胞术对Ba P作用后的G0/G1期和S期细胞进行分选,Western blot方法分析相关蛋白表达水平的变化。结果Ba P作用HELF后,在总细胞群中和分选获得的S期细胞中均发现Chk1磷酸化水平升高,Cdc25A的表达水平降低;只在分选获得的S期细胞中,发现Cyclin E表达水平降低,CDK2和p53的表达水平升高。结论 Ba P通过启动Chk1-Cdc25A和p53两条信号转导通路共同作用于Cyclin E/CDK2,从而引发HELF细胞发生S期阻滞。Objective To observe the cell cycle proteins of arrested S phase cells in human embryo lung fibroblasts cells（HELF）caused by benzo（a） pyrene（BaP） and to provide clues to explore molecular mechanism of carcinogenesis induced by BaP. Methods After exposure with 5 μmol/L BaP for 4 h,the HELF cells were harvested for cell cycle sort and western blot analysis at 24 h. Results After treatment of BaP,phosphorylated Chk1 level increased and Cdc25 A expression level decreased in both total cell group and the sorted S phase cell group. However,we only observed that Cyclin E decreased and CDK2 and p53 increased in the sorted S phase cell group. Conclusion The co-effect of Chk1-Cdc25 A and p53 on Cyclin E/CDK2 compound participated in the S-phase arrest induced by BaP in HELF cells.

关 键 词：苯并(A)芘 细胞分选 S期阻滞 

分 类 号：R994.6[医药卫生—毒理学]