姜黄素对人肝癌细胞SMCC-7721增殖及干细胞标志物表达的影响及机制  

Effects of curcumin on human hepatocellular carcinoma SMCC-7721 cells proliferation and stem cell markers expression and its mechanism

作　　者：王斌[1] 潘战宇[1] 闫祝辰[1] 刘东颖[1] 兰岚[1] 王琮[1] 姜战胜[1] 

机构地区：[1]天津医科大学肿瘤医院中西医结合科,天津300060

出　　处：《河北中医》2016年第12期1778-1783,共6页Hebei Journal of Traditional Chinese Medicine

基　　金：天津医科大学肿瘤医院科研项目基金(编号:1320)

摘　　要：目的观察姜黄素对人肝癌细胞SMCC-7721增殖及干细胞标志物表达的影响,并探讨其相关机制。方法实验分为对照组、姜黄素组、AG490组、姜黄素+AG490组,其分别作用于人肝癌细胞SMCC-7721后,通过噻唑蓝(MTT)法分别检测其对人肝癌细胞SMCC-7721生长的抑制作用;通过Western blot法、RT-PCR法分别检测各组人肝癌细胞SMCC-7721中转录激活因子3(STAT3)、p-STAT3、CD24、CD133、CD44和CD90蛋白的表达及mRNA的变化。结果姜黄素可明显抑制人肝癌细胞SMCC-7721的增殖,同时下调STAT3、p-STAT3、CD24、CD133、CD44和CD90蛋白表达;其作用强度较AG490稍弱,但两药合用显示出一定的协同效果。结论姜黄素抑制人肝癌细胞SMCC-7721的增殖,降低人肝癌细胞SMCC-7721中CD24、CD133、CD44和CD90表达,其机制可能与调控STAT3磷酸化相关。Objective To observe the effects of curcumin on the human hepatocellular carcinoma SMCC - 7721 cells proliferation and stem cell markers expression and to investigate its related mechanism. Methods The experiment includes blank group, curcumin group, JAK2 kinase inhibitor AG490 group and curcumin ＋ AG490 group. The human hepatocellu^ar carcinoma SMCC -7721 cells in each group received corresponding intervention. Colorimetric MTT assay was used to assess the inhibitory effect of curcumin, AG490, and their combination on the proliferation of human hepatocellular carcinoma SMCC - 7721 cells. The expressions the protein and mRNA of STAT3, p - STAT3, CD24, CD133, CD44 and CD90 were measured by real time RT - PCR and western blot in hu- man hepatocellular carcinoma SMCC - 7721 cells. Results Curcumin inhibited the proliferation of SMCC - 7721 cells and downregulated the protein expression of STAT3, p - STAT3, CD24, CD133, CD44 and CD90. Its action was slightly weaker than AG490; but their combination showed a synergistic effect. Conclusion Curcumin inhibits the proliferation of SMCC - 7721 cells, which involves curcumin - induced decline in the expression of CD24, CD133, CD44 and CD99 by repressing STAT3 phosphorylation.

关 键 词：姜黄素 肝肿瘤 基因表达 细胞凋亡 中药疗法 

分 类 号：R282.71[医药卫生—中药学] R735.7[医药卫生—中医学]