以脑能量转运蛋白为靶点研究通络醒脑泡腾片的抗痴呆作用  

Anti Dementia Effects of Tongluo Xingnao Effervescent Tablet With the Target of Brain Energy Transporter

作　　者：付文君[1,2] 胡勇[1,2] 任香怡 魏江平[1,2] 付昆[1,2] 徐世军[1,2] 

机构地区：[1]四川省中药资源系统研究与开发利用国家重点实验室培育基地,成都611137 [2]成都中医药大学,成都611137

出　　处：《中国中医基础医学杂志》2016年第12期1612-1615,1651,共5页JOURNAL OF BASIC CHINESE MEDICINE

基　　金："重大新药创制"科技重大专项(2013ZX09103002-008)-脑络重塑治疗老年痴呆新药-通络醒脑微丸的研究;四川省杰出青年学术技术带头人计划项目(2011JQ0014)-芩芎当归解毒益智方对Aβ代谢的调控及机制研究;成都市产业发展技术支撑计划-高校院所应用基础研究项目(12DXYB321JH-002)-通络醒脑泡腾片抗老年痴呆的代谢组学及改善认知的机构基础研究

摘　　要：目的:通过考察通络醒脑泡腾片干预后MID模型大鼠海马主要能量转运蛋白表达揭示其抗痴呆的作用机制。方法:建立微血栓诱发的MID大鼠模型,给予通络醒脑泡腾片干预90 d,采用Morris水迷宫评价大鼠的学习记忆功能,采用免疫组化和图像分析法评价大鼠海马GLUT-1、MCT-1和MCT-2的表达。结果:与模型对照组比较,通络醒脑泡腾片能显著缩短MID模型大鼠的逃避潜伏期,增加进入有效区域次数并延长有效区域停留时间,差异有统计学意义;通络醒脑泡腾片能显著促进模型大鼠海马GLUT-1、MCT-1和MCT-2表达。结论:通络醒脑泡腾片改善MID模型大鼠学习记忆功能的作用与其促进增强海马能量转运蛋白GLUT-1、MCT-1和MCT-2的表达有关。Objective： To study the effect of Tongluoxingnao effervescent tablet（TLXNET） on the expression of energy transport-related proteins in multi-infarct dementia（MID） rats in order to explore its mechanism of anti-dementia.Methods： Microthrombus was used to establish MID model rats which were intervened by gastric infusion of TLXNET for 90 d. Evalneting the learning and memory ability of MID model rats via Morris water maze. Additionally the expressions of GLUT-1,MCT-1 and MCT-2 in hippocampal were measured by immunohistochemical and image analysis. Results：Compared with the model control group,TLXNET could significantly reduce the escape latency,increase times of entrance into the valid region and prolong the residence time of MID model rats,which all revealed a significant statistic; meanwhile the expressions of GLUT-1,MCT-1 and MCT-2 were notably raised after administration of TLXNET,with an increase in the average optical density. Conclusion： TLXNET can improve learning and memory deficit which is related with promoting the expression of energy metabolism-related proteins transportation such as GLUT-1,MCT-1 and MCT-2.

关 键 词：通络醒脑泡腾片 多发脑梗死性痴呆 葡萄糖转运体-1 单羧酸转运体-1 单羧酸转运体-2 

分 类 号：R285.5[医药卫生—中药学]