一测多评法同时测定北青龙衣药材中胡桃醌和胡桃酮的含量  

Simultaneous Determination of Contents of Juglone and Regiolone in Juglans mandshurica by Quantitative Analysis of Multi-components by Single-maker

作　　者：霍金海 张海燕 王伟明 

机构地区：[1]黑龙江省中医药科学院中药研究所,哈尔滨150036 [2]黑龙江省林下经济资源研发与利用协同创新中心,哈尔滨150040

出　　处：《中国药房》2017年第3期380-383,共4页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81503348);国家"重大新药创制"科技重大专项课题(No.2009ZX09102-138)

摘　　要：目的:建立同时测定北青龙衣药材中胡桃醌、胡桃酮含量的方法。方法:采用高效液相色谱法,以胡桃醌为内参物,计算其对胡桃酮的相对校正因子(RCF),通过RCF计算北青龙衣药材中胡桃酮的含量;以外标法测定的胡桃酮含量作实测值,采用夹角余弦法比较一测多评法的计算值与实测值的差异。结果:胡桃醌、胡桃酮检测质量浓度线性范围分别为7.6~76.0μg/mL(r=0.999 0)、8.2~82.0μg/mL(r=0.999 4);精密度、稳定性、重复性试验的RSD<3.0%;加样回收率分别为95.37%~97.94%(RSD=1.04%,n=6)、99.13%~100.10%(RSD=0.33%,n=6)。计算值与实测值之间的夹角余弦值为0.999 84,两者差异无统计学意义(P>0.05)。结论:该方法操作简便,精密度、稳定性、重复性好,可用于北青龙衣药材中胡桃醌、胡桃酮含量的同时测定。OBJECTIVE： To establish a method for the simultaneous determination of juglone and regiolone in duglans mandsh- urica. METHODS： HPLC was used and regiolone was used as internal reference, the relative correction factor （RCF） of regiolone and juglone wascalculated, then calculated the content of juglone based on RCF; the juglone content was used as measured value, which was determined by external standard method, angle cosine method was used to compared the difference of calculated value in quantitative analysis of multi-components by single-maker and measured value in external standard method. RESULTS： The linear range was 7.6-76.0μg/mL for juglone （r=0.999 0） and 8.2-82.0 μg/mL for regiolone （r=0.999 4）; RSDs of precision, stabili- ty and reproducubility tests were lower than 3.0%; recoveries were 95.37%-97.94% （RSD=1.04%, n=6） and 99.13%-100.10% （RSD=0.33% ,n=6）. The cosine of the included angle between the calculated and measured values was 0.999 84, and there was no significant difference （P〉0.05）. CONCLUSIONS： The method is simple with good precision, stability and reproducibility, and can be used for the simultaneous determination ofjuglone and regiolone in J. mandshurica.

关 键 词：北青龙衣 一测多评 高效液相色谱法 校正因子 胡桃醌 胡桃酮 含量 

分 类 号：R284.1[医药卫生—中药学]