HPLC法同时测定软肝缩脾丸中芍药苷和丹酚酸B的含量  

Simultaneous Determination of Paeoniflorin and Salvianolic Acid B in Ruangan Suopi Pill by HPLC

作　　者：刘栋 宋晓红 汤长明 王丽军 张建邦 

机构地区：[1]解放军第一五三中心医院制剂室,郑州450042

出　　处：《中国药房》2017年第3期416-418,共3页China Pharmacy

摘　　要：目的:建立同时测定软肝缩脾丸中芍药苷和丹酚酸B含量的方法。方法:采用高效液相色谱法。色谱柱为Wondasil C_(18),流动相为乙腈-0.1%磷酸溶液(梯度洗脱),流速为1.0 mL/min,检测波长为230 nm(芍药苷)、286 nm(丹酚酸B),柱温为30℃,进样量为10μL。结果:芍药苷和丹酚酸B检测质量浓度线性范围分别为9.79~195.80μg/mL(r=0.999 9)、11.45~229.00μg/mL(r=0.999 9);定量限分别为0.018 1、0.014 4μg,检测限分别为0.005 4、0.004 3μg;精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为98.67%~102.22%(RSD=1.26%,n=9)、99.72%~103.28%(RSD=1.05%,n=9)。结论:该方法快速、灵敏、准确,可用于软肝缩脾丸中芍药苷和丹酚酸B含量的同时测定。OBJECTIVE： To establish a method for simultaneous determination of paeoniflorin and salvianolic acid B in Ruan- gan suopi pill. METHODS： HPLC was performed on the column of Wondasil C18 with mobile phase of acetonitrile -0.1% phosphor- ic acid solution （gradient elution） at a flow rate of 1.0 mL/min, the detection wavelength was 230 nm for paeoniflorin and 286 nm for salvianolic acid B, the column temperature was 30 ℃, and the injection volume was 10 μL. RESULTS：The linear range was 9.79-195.80 μg/mL（r=0.999 9） for paeoniflorin, 11.45-229.00 μg/mL（r=0.999 9） for salvianolic acid B; the limits of quantifica- tion were 0.018 1,0.014 4 μg, limits of detection were 0.005 4, 0.004 3 μg; RSDs of precision, stability and reproducibility tests were lower than 2.0% ; the recoveries were 98.67%-102.22% （RSD= 1.26%, n=9） and 99.72%-103.28% （RSD= 1.05%, n=9）. CONCLUSIONS： The method is rapid, sensitive and accurate, and can be used for the simultaneous determination of paeoniflorin and salvianolic acid B in Ruangan suopi pill.

关 键 词：软肝缩脾丸 芍药苷 丹酚酸B 含量测定 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]