Toll样受体7、9及其调控分子MyD88、NF—κB在白癜风患者外周血单个核细胞中的表达  

Expression of Toll-like receptors 7 and 9 as well as their regulatory molecules myeloid differentiation factor 88 and nuclear factor-κB in peripheral blood mononuclear cells of patients with vitiligo

作　　者：唐亚平[1] 盛文婷[2] 周欣[1] 黎小东[1] 田歆[1] 钟金宝[1] 李振洁[1] 林春生[1] 刘玉梅[1] 

机构地区：[1]广州市皮肤病防治所皮肤科,510095 [2]广州市红十字会医院皮肤科

出　　处：《中华皮肤科杂志》2017年第1期13-17,共5页Chinese Journal of Dermatology

基　　金：广东省科技计划项目（2012A030400050）;广州市医药卫生科技项目（20151A011063）

摘　　要：目的探讨Toll样受体7、9（TLR7、TLR9）及调控分子MyD88、NF-κB在白癜风患者以及健康个体外周血单个核细胞（PBMC）中的表达差异及意义。方法用流式细胞仪检测36例白癜风患者及22例健康对照PBMC中TLR7、TLR9的表达，并用反转录聚合酶链反应（RT—PCR）检测上述样本中MyD88、NF-κBmRNA的表达。结果白癜风患者TLR7的表达高于健康对照组，但差异无统计学意义（t=1．477，P=0．145）；TLR9的表达低于健康对照组，差异无统计学意义（t=-1．761，P=0．084）。白癜风患者外周血PBMC中MyD88mRNA的表达稍高于对照组，但差异无统计学意义（t=0．058，P=0．954）；NF-κBmRNA的表达明显高于对照组，差异有统计学意义（t=2．814，P=0．008）。结论TLR7、TLR9的调控通路中的关键信号分子NF-κB表达升高，提示NF-κB可能参与白癜风的发病机制。Objective To compare expression of Toll-like receptors 7 and 9 （TLR7, TLR9） as well as their regulatory molecules myeloid differentiation factor 88 （MyD88） and nuclear factor-KB （NF-κB） in peripheral blood mononuclear cells （PBMCs） between patients with vitiligo and healthy individuals, and to explore their significance. Methods Flow cytometry was performed to measure expression of TLR7 and TLR9 in PBMCs among 36 patients with vitiligo and 22 healthy controls, and real-time fluorescencebased quantitative PCR （RT-PCR） was conducted to determine mRNA expression of MyD88 and NF-κB in the above blood samples. Results Compared with healthy controls, patients with vitiligo showed higher expression of TLR7 and mRNA expression of MyD88 and NF-κB, but lower expression of TLR9. However, significant differences were only observed in the mRNA expression of NF-κB （t = 2.814, P = 0.008）, but not in the expression of TLR7 and TLR9 or the mRNA expression of MyD88 between patients and controls （t = 1.477, 1.761, 0.058, all P 〉 0.05）. Conclusion NF-κB, as a key signaling molecule of TLR7 and TLR9 regulation pathways, increases obviously in patients with vitiligo, suggesting that NF-κB may be involved in the pathogenesis of vitiligo.

关 键 词：白癜风 TOLL样受体7 TOLL样受体9 髓样分化因子88 NF-κB 

分 类 号：R758.41[医药卫生—皮肤病学与性病学]