白癜风患者血清中抗酪氨酸酶IgG抗体及抗酪氨酸酶相关蛋白1IgG抗体的检测  

Detection of anti-tyrosinase IgG antibody and anti- tyrosinase-related protein-1 IgG antibody in sera of patients with vitiligo

作　　者：韩莹[1] 李铁男[1] 刘文力[1] 金春琳[1] 程欣[1] 刘奕 贺莎 吴善东 

机构地区：[1]沈阳市第七人民医院皮肤科,110003 [2]杭州浙大迪迅生物基因工程有限公司

出　　处：《中华皮肤科杂志》2017年第1期48-50,共3页Chinese Journal of Dermatology

基　　金：沈阳市科技计划项目（F14-158-9-10）

摘　　要：目的探讨白癜风患者血清中抗酪氨酸酶IgG抗体（TYRIgG）和抗酪氨酸酶相关蛋白1IgG抗体（TRP-1IgG）与白癜风的关系。方法用酶联免疫吸附测定检测260例白癜风患者和50例健康对照血清中TYR1gG和TRP-1IgG的含量。结果白癜风组TYRIgG和（或）TRP-1IgG阳性率57．31％（149／260），TYRIgG阳性率37．3％（97／260），TRP-1IgG阳性率33．5％（87／260），均明显高于对照组（r=25．441、21．630，P〈0．01）。TYRIgG阳性与TRP-1IgG阳性不相关（r=-0．032，P〉0．05）。白癜风组TYRIgG阳性率男女间差异无统计学意义（P〉0．05），但TRP-1IgG阳性率女性高于男性（r=5．811，P〈0．05）。白癜风TYRIgG阳性率≤20岁（35．9％）与〉20岁（38.3％）比较，差异无统计学意义（P〉0．05），而TRP-1IgG阳性率≤20岁组明显高于〉20岁组（x^2=6．498，P〈0．05）。结论检测TYRIgG和TRP-1IgG可为白癜风的诊断和治疗提供一定依据。Objective To investigate relationships between serum levels of anti-tyrosinase IgG antibody （TYR IgG） as well as anti-tyrosinase-related protein-1 IgG antibody （TRP-1 IgG） and vitiligo. Methods Enzyme linked immunosorbent assay （ELISA） was performed to detect serum levels of TYR IgG and TRP-1 IgG in 260 patients with vitiligo and 50 health controls. The threshold for defining a positive test result was set at 3 standard deviations above the mean serum level of TYR IgG or TRP- 1 IgG in the healthy controls. Results The positive rate of TYR IgG and/or TRP-1 IgG in the vitiligo group was 57.31% （149/260）. The positive rates of TYR IgG and TRP- 1 IgG were both significantly higher in the vitiligo group than in the control group （TYR IgG： 37.3%[97/260] vs. 0, ff = 25.441, P 〈 0.01; TRP-1 IgG： 33.5% [ 87/260 ] vs. 0, x^2 = 21.630, P 〈 0.01 ）. The positive rate of TYR IgG was not associated with that of TRP-1 IgG in the vitiligo group （r = -0.032, P 〉 0.05）. Among patients with vitiligo, the positive rate of TRP-1 IgG was significantly higher in females than in males （x^2 = 5.811, P 〈 0.05）, as well as in patients aged ≤ 20 years than in those aged 〉 20 years （A2 = 6.498, P 〈 0.05）, while the positive rate of TYR IgG didn＇t differ between females and males, or between patients aged ≤ 20 years and those aged 〉 20 years （both P 〉 0.05）. Conclusion Detection of TYR IgG and TRP-1 IgG may provide some evidence for the diagnosis and treatment of vitiligo.

关 键 词：白癜风 一元酚单氧酶 女性 青少年 抗酪氨酸酶IgG抗体 抗酪氨酸酶相关蛋白-1IgG抗体 

分 类 号：R758.41[医药卫生—皮肤病学与性病学]