TLR3-1377C/T基因多态性及表达水平与儿童肠道病毒71型脑炎易感性的探讨  

Association of TLR3-1377C/T gene polymorphisms and expression with susceptibility to enterovirus 71 encephalitis in children

作　　者：苑爱云[1] 何红芳 吕富岩[1] 刘培培[2] 胡静飞[3] 陈宗波[2] 

机构地区：[1]青岛大学附属青岛妇女儿童医院神经康复科,山东青岛266034 [2]青岛大学附属医院儿科,山东青岛266003 [3]青岛大学附属青岛妇女儿童医院新生儿科,青岛266034

出　　处：《中国当代儿科杂志》2017年第1期39-43,共5页Chinese Journal of Contemporary Pediatrics

基　　金：国家自然科学基金(31171212)

摘　　要：目的研究Toll样受体(TLRs)基因TLR3-1377C/T位点基因多态性及TLR3表达水平与儿童肠道病毒71型(EV71)脑炎易感性之间的关系。方法收集EV71感染患儿187例(脑炎组59例和无脑炎组128例)与同期健康体检儿童232例进行病例对照研究。采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)方法对TLR3-1377C/T基因多态性进行检测,采用ELISA检测血清TLR3水平。结果与EV71感染无脑炎组相比,脑炎组TLR3-1377C/T位点基因型分布及等位基因频率的差异无统计学意义(P>0.05)。与对照组相比,EV71感染脑炎组、无脑炎组的血清TLR3水平均显著增高(P<0.05),以无脑炎组最高(P<0.05)。脑炎组的EV71病毒载量高于无脑炎组(P<0.01)。<1岁或≥1岁的EV71感染脑炎组、无脑炎组患儿的血清TLR3水平均较相应对照组增高(P<0.05),其中无脑炎组TLR3水平高于相应年龄的脑炎组(P<0.05)。脑炎组≥1岁患儿的TLR3浓度高于<1岁者(P<0.05);无脑炎组及对照组的TLR3浓度在<1岁或≥1岁患儿之间的差异无统计学意义(P>0.05)。EV71感染脑炎组,<1岁患儿所占比例高于≥1岁者(P<0.05)。结论 TLR3-1377C/T位点的基因多态性与EV71脑炎的发生无明显相关性。TLR3的低表达可能导致对病毒复制的抑制作用减弱,促进了EV71脑炎的发生。婴儿EV71感染后血清TLR3的表达不足可能是其合并脑炎的重要因素。Objective To investigate the association of gene polymorphisms of Toll-like receptor 3（TLR3）-1377C/T and expression of TLR3 with the susceptibility to enterovirus 71（EV71） encephalitis in children. Methods A total of 187 children with EV71 infection（59 children in the encephalitis group and 128 in the non-encephalitis group） and 232 children who underwent physical examination were enrolled in the case-control study. Polymerase chain reactionrestriction fragment length polymorphism was used to detect the TLR3-1377C/T gene polymorphisms. ELISA was used to measure the serum level of TLR3. Results There were no significant differences in the genotype and allele frequencies of TLR3-1377C/T between the non-encephalitis group and the encephalitis group. Compared with the control group, the encephalitis group and the non-encephalitis group had significant increases in the serum level of TLR3（P0.05）, and the non-encephalitis group had the highest level（P0.05）. The encephalitis group had a significantly higher EV71 viral load than the non-encephalitis group（P0.01）. The children aged 1 year or ≥1 year in the encephalitis group and the non-encephalitis group had significant increases in the serum level of TLR3 compared with their counterparts inthe control group（P0.05）, and the children aged 1 year or ≥1 year in the non-encephalitis group had a significantly higher serum level of TLR3 than those in the encephalitis group（P0.05）. In the encephalitis group, the children aged ≥1 year had a significantly higher TLR3 concentration than those aged 1 year（P0.05）, and there were no significant differences in the TLR3 concentration between the children aged ≥1 year and 1 year in the non-encephalitis group and the control group. In the encephalitis group, the proportion of children aged 1 year was significantly higher than those aged ≥1 year（P0.05）. Conclusions The TLR3-1377C/T gene polymorphisms are not significantly associated with the development of EV71 encephalitis. Low expression

关 键 词：肠道病毒71型 脑炎 TLR3 单核苷酸多态性 儿童 

分 类 号：R725.1[医药卫生—儿科]