腹泻病婴幼儿艰难梭菌定植与感染情况分析  

The analysis of clostridium difficile colonization and infection in infants with diarrhea

作　　者：郭亚楠[1] 王君[1] 李佳垚 王秋萍[1] 刘欣竹[1] 

机构地区：[1]中日友好医院儿科,北京100029 [2]北京协和医院检验科,北京100073

出　　处：《中国中西医结合儿科学》2016年第6期607-609,共3页Chinese Pediatrics of Integrated Traditional and Western Medicine

摘　　要：目的 了解腹泻病婴幼儿艰难梭菌定植与感染情况并探讨其检测方法。方法 收集2014~2015年儿科消化门诊腹泻婴幼儿的临床资料及粪便,通过厌氧培养、毒素A和B PCR扩增法和酶联免疫法检测粪便中艰难梭菌和毒素。结果 65份标本中,厌氧培养阳性19例（29.23%）,19例经PCR扩增,A/B毒素阳性5例（7.69%）;酶联免疫法检测抗原阳性22例（33.85%）,与厌氧培养比较,差异无统计学意义（P〉0.05）,毒素A/B阳性5例（7.69%）与PCR扩增法比较,差异无统计学意义（P〉0.05）。结论 婴幼儿有较高的艰难梭菌定植率,其中多为非产毒株,并不致病;酶联免疫法检测艰难梭菌抗原和毒素可以用于临床筛检。Objective To investigate clostridium difficile bacteria colonization and infection in infants with diarrhea and discuss the testing methods.Methods The cases and feces of infants with diarrhea were collected in pediatric digestive outpatient from 2014 to 2015.We detected clostridium difficile bacteria and toxins in the feces by anaerobic culture,toxin A and B PCR amplification method,and enzyme-linked immu-nosorbent assay.Results Of 65 specimens,19 cases（29.2%）were positive for anaerobic culture.The A/B toxin were positive in 5 cases（6.2%）in the 19 cases by PCR amplification.Antigen was positive in 22 cases （33.8%）by enzyme-linked immunoassay detection,and there was no statistically significant difference when compared with anaerobic culture.Toxin A/B was positive in 5 cases（6.2%）,and there was no statistically significant difference when compared with PCR amplification method.Conclusion Infants have higher clos-tridium difficile colonization rate,and the majority does not produce toxins and does not cause diseases.En-zyme-linked immunoassay detection for clostridium difficile antigen and toxins can be used for clinical screening.

关 键 词：腹泻 艰难梭菌 婴幼儿 

分 类 号：R723.11[医药卫生—儿科]