舒芬太尼对小鼠周围神经损伤后再生和修复的影响  

Effects of sufentanil on regeneration and repair after peripheral nerve injury in mice

作　　者：李海波[1] 孙义[1] 周琪[1] 曹剑[1] 梁晓东[1] 宋健楠[1] 毕立伟 张析哲[1] 

机构地区：[1]内蒙古自治区赤峰市医院麻醉科,024000

出　　处：《中华麻醉学杂志》2016年第11期1353-1355,共3页Chinese Journal of Anesthesiology

摘　　要：目的评价舒芬太尼对小鼠周围神经损伤后再生和修复的影响。方法健康雄性BALB/c小鼠75只，体重18～22 g，6～8周龄，采用随机数字表法分为5组（n＝15）：周围神经损伤组（PNI组）、低剂量、中剂量、高剂量舒芬太尼组（L组、M组和H组）和环孢素A组（C组），5组均建立小鼠单侧坐骨神经损伤模型。L组、M组和H组造模后分别腹腔注射舒芬太尼2.5、5.0、10.0 μg/kg，C组给予环孢素A 50 mg/kg，PNI组给予等容量生理盐水，1次/d，连续3 d。分别于术后4、8、12周时进行神经电生理监测，记录坐骨神经复合肌肉动作电位波幅，并计算其神经传导速度。于术后8周时处死5只小鼠，取吻合口上下0.5 cm坐骨神经，光学显微镜下观察髓鞘形态学，计算神经纤维的数量。结果与PNI组比较，M组、H组和C组坐骨神经复合肌肉动作电位波幅、神经传导速度和神经纤维数量增加（P〈0.05），L组上述指标比较差异无统计学意义（P〉0.05）；PNI组髓鞘排列严重不规则，空泡较多；L组髓鞘排列不规则，空泡较多；M组髓鞘排列大部分规则，偶有空泡；H组和C组髓鞘排列规则，未见空泡。结论舒芬太尼可促进小鼠周围神经损伤后再生和修复。ObjectiveTo evaluate the effects of sufentanil on regeneration and repair after single sciatic nerve injury in mice.MethodsSeventy-five healthy male BALB/c mice, aged 6-8 weeks, weighing 18-22 g, were divided into 5 groups （n=15 each） using a random number table： peripherial nerve injury group （group PNI）, low, medium and high doses of sufentanil groups （L, M and H groups） and cyclosporine A group （group C）. The model of unilateral sciatic nerve transection was established in the 5 groups.In L, M and H groups, sufentanil 2.5, 5.0 and 10.0 μg/kg were injected intraperitoneally, respectively, once a day for 3 consecutive days.Cyclosporine A 50 mg/kg was injected intraperitoneally once a day for 3 consecutive days in group C. The equal volume of normal saline was given once a day for 3 consecutive days in group PNI.Neurophysiological monitoring was performed at 4, 8 and 12 weeks after operation, the amplitude of compound muscle action potentials of the sciatic nerve was recorded, and the nerve conduction velocity was measured.At 8 weeks after operation, 5 mice were sacrificed, the sciatic nerve 0.5 cm of the upper and lower the anastomosed site was removed for examination of the morphology of myelin sheath with light microscope, and the number of nerve fibers was calculated.ResultsCompared with group PNI, the amplitude of compound muscle action potentials of the sciatic nerve, nerve conduction velocity and the number of nerve fibers was were significantly increased in M, H and C groups （P〈0.05）, and no significant change was found in the parameters mentioned above in group L （P〉0.05）. Myelin sheath arrangement was severely irregular, and more vacuoles were found in group PNI.Myelin sheath arrangement was irregular, and more vacuoles were found in group L. Myelin sheath arrangement was mainly regular, and vacuoles were found occasionally in group M. Myelin sheath arrangement was regular, and no vacuoles were observed in H and C groups.ConclusionSufentanil can promote regeneration and re

关 键 词：舒芬太尼 周围神经 神经再生 

分 类 号：R614[医药卫生—麻醉学]