鸭IL-2基因启动子的克隆、多态性与表达的关联分析  

Cloning of duck IL-2 gene promoter and correlation analysis about polymorphism in the promoter region with its expression levels

作　　者：张涛[1] 刘贺贺[1] 罗俊[1] 李亮[1] 黄惠兰[1] 何桦[1] 

机构地区：[1]四川农业大学畜禽遗传资源发掘与创新利用四川省重点实验室,四川成都611130

出　　处：《浙江农业学报》2016年第12期1985-1991,共7页Acta Agriculturae Zhejiangensis

基　　金：四川省科技支撑项目(2015JY0110)

摘　　要：为研究鸭IL-2基因调控区单核苷酸多态性对其转录调控的影响,克隆获得了鸭IL-2基因启动子2850 bp序列,与人、小鼠和原鸡的同源性分别为35.37%,37.52%和34.74%。其中,-1 400/-1 000存在集中的核心转录因子结合位点。对鸭IL-2基因启动子(-1 932/-742)进行单核苷酸多态检测和遗传多态性分析发现,该区域存在两个突变位点(C-1353A、C-1406T),且均处于Hardy-Weinberg极不平衡状态;等位基因A均为优势等位基因。单核苷酸多态与其表达水平的相关性分析发现,突变位点不同基因型与IL-2基因mRNA表达水平和IL-2蛋白水平均无显著相关关系,但基因型AA个体的mRNA表达量均高于其他基因型个体(P>0.05),表明鸭IL-2启动子等位基因A可能有促进IL-2基因转录的趋势。研究结果为IL-2基因转录调控机制的研究提供了理论基础。To study the effect of single nucleotide polymorphism（SNP） of duck IL-2 gene regulatory region on transcriptional regulation,a 2 850 bp promoter sequence of duck IL-2 gene was obtained by cloning. The homology analysis showed that the homology of duck IL-2 promoter sequence with human,mouse and chicken was 35. 37%,37. 52% and 34. 74%,respectively. The core transcription factor binding sites was concentrated at the region（-1400/-1000）. The detecting of SNP and genetic polymorphism of duck IL-2 gene promoter fragment（-1932/-742） found two polymorphism sites（C-1406 T and C-1353A）. Allele A was the predominant allele,and both polymorphism sites were not in Hardy-Weinberg. Correlation analysis showed that the mRNA expression levels of IL-2gene and IL-2 protein levels had no significant relationship with different genotypes of the polymorphism sites. However,the expression levels of IL-2 mRNA in individuals with genotype AA were higher than those in other genotypes（P〉0. 05）,suggesting that the allele A might promote the transcription of IL-2 gene. The results provided a theoretical basis for studies on the transcription regulation mechanism of IL-2 gene.

关 键 词：鸭 IL-2 单核苷酸多态 转录调控区 转录水平 

分 类 号：S834[农业科学—畜牧学] Q754[农业科学—畜牧兽医]