均匀设计优化超临界CO_2流体提取桑叶黄酮的研究  

Optimization processing of super-critical CO_2 extraction of total flavonoids from mulberry leaves using uniform design method

作　　者：田刚[1] 杨丛[2] 赵晓军[1] 彭效明[3] 李松田[1] 

机构地区：[1]平顶山学院化学与环境工程学院,河南平顶山467000 [2]平顶山学院数学与统计学院,河南平顶山467000 [3]北京石油化工学院化学工程学院,北京102617

出　　处：《农业科学研究》2016年第4期23-26,35,共5页Journal of Agricultural Sciences

基　　金：平顶山学院高层次人才科研启动经费资助(PXY-BSQD-2015007);国家青年科学基金项目(21406015)

摘　　要：利用均匀设计法对超临界CO_2流体提取桑叶黄酮的工艺进行优化,主要考察提取温度、提取压力、提取时间和夹带剂乙醇用量对桑叶黄酮提取率的影响.结果表明:超临界CO_2流体提取桑叶黄酮的最佳提取条件为提取温度51℃,提取压力36 MPa,提取时间4.0 h,夹带剂乙醇用量3.7 m L/g.实际测得的桑叶黄酮的提取率为1.42%,与模型预测值相符,说明采用均匀设计法优化超临界CO_2流体提取桑叶黄酮的工艺准确可靠.此外,与回流提取法、超声波辅助提取法相比,超临界CO_2提取法具有提取温度低、溶剂使用少、提取率相对较高的特点,适于桑叶黄酮提取.Uniform design methodology for the extraction process of flavonoids of mulberry leaves by super-critical CO2fluid（SCF） was studied. The effects of extraction temperature, extraction pressure, extraction time and the amount of ethanol entrainer on the yields of flavonoids were investigated. The results show that the optimum extraction conditions of flavonoids of mulberry leaves were extraction temperature 51 ℃, extraction pressure 36 MPa, extraction time 4.0 h, amount of ethanol entrainer 3.7 m L·g-1. The actual measured the yields of flavonoids is 1.42% and is basically consistent with the predicted value. These show that using uniform design methodology to model the extraction process of flavonoids of mulberry leaves by SCF was accurate and reliable. Additionally, compared with the reflux extraction and the ultrasonic-assisted extraction, SCF extraction achieved relatively acceptable extraction efficiency using less ethanol solvent at a lower extraction temperature, is a suitable method to extract flavonoids of mulberry leaves.

关 键 词：桑叶 黄酮 超临界CO2流体提取 均匀设计 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]