芒萁组织培养和快繁技术研究  

Study on Tissue Culture and Rapid Propagation Technique of Dicranopteris dichotoma Hance Meristem

作　　者：杨春梅[1,2] 屈云慧[1] 汪国鲜[1,2] 单芹丽[1,2] 吴丽芳[1,2] 阮继伟[1,2] 蒋海玉[3] 

机构地区：[1]云南省农业科学院花卉研究所云南省花卉育种重点实验室,云南昆明650205 [2]玉溪云星生物科技有限公司,云南玉溪653200 [3]云南省农业科学院农业经济信息研究所,云南昆明650205

出　　处：《西南农业学报》2016年第12期2955-2958,共4页Southwest China Journal of Agricultural Sciences

基　　金：云南省技术创新暨产业发展专项资金(2015XB015)

摘　　要：以孢子为外植体对芒萁进行组织培养。在基本培养基中添加不同生长调节物质对孢子萌发和孢子体进行诱导。结果表明，孢子体萌发阶段最适培养基组合是1／2MS培养基＋30g／L蔗糖＋7g／L琼脂，pH值5．8～6．0；原叶体增殖阶段，最佳培养基组合为1／2MS＋NAA1．0mg／L＋6-BA1．5mg／L＋蔗糖30∥L＋琼脂7g／L；孢子体的诱导最适培养基组合是1／2MS培养基＋NAA0．1mg／L＋蔗糖30g／L＋琼脂7g／L。The micropmpagation system on Dicranopteris dichotoma with spore asexplants was established. The spore bourgeon and sporophyte induction was studied through adding different growth material in minimal medium. The optimum culture medium for spores bourgeon was 1/ 2 MS ＋ sucrose 30 g/L ＋ agar 7 g/L,and pH value was 5.8 -6. 0. The optimum culture medium for prothallus proliferation was 1/2 MS ＋ NAA 1.0 mg/L ＋ 6-BA 1.5 mg/L ＋ sucrose 30 g/L ＋ agar 7 g/L. And the optimum eulture for sporophyte inductio was 1/2 MS ＋ NAA0.0 mg/L ＋ sucrose 30 g/L ＋ agar 7 g/L.

关 键 词：芒萁 组织培养 快繁技术 孢子体诱导 

分 类 号：S682.35[农业科学—观赏园艺]