产黄曲霉毒素真菌多重PCR检测体系的建立  

Establishment of Multiplex PCR for the Detection of Aflatoxigenic Fungi

作　　者：李慧 蔡军 胡梦龙 傅洋 欧静堃 

机构地区：[1]中粮营养健康研究院 [2]营养健康与食品安全北京市重点实验室,北京102209

出　　处：《中国粮油学报》2017年第1期125-129,共5页Journal of the Chinese Cereals and Oils Association

基　　金：国家科技支撑计划(2012BAK08B04-01)

摘　　要：依据标准SN/T 2582—2010《产黄曲霉毒素真菌PCR检测方法》推荐的产黄曲霉毒素调节基因afl R、柄曲霉素转甲氧基酶基因omt-1、杂色曲霉素A脱氢酶基因ver-1及真菌共有的5.8S r DNA的ITS序列分别设计4对特异性引物,对4对引物进行多重PCR反应体系构建与优化,建立快速检测产黄曲霉毒素真菌的多重PCR方法,并应用10株试验菌株对所建立的检测方法进行验证。结果表明,建立的多重PCR方法具有灵敏度高、特异性强、方便快捷的优点,为产黄曲霉毒素真菌快速检测试剂盒的研发及应用提供了重要技术保障。To establish a multiplex PCR method for rapid and high sensitivity detection of aflatoxigenic fungi, 4 pairs of specific primers were designed according to the specific genes recommended by the standard of SN/T 2582-- 2010 Detection of aflatoxigenic strains of Aspergillus by PCR, including aflatoxin biosynthesis regulatory gene aflR, ver- sicolorin A dehydrogenase gene vet - 1, sterigmatocystin o - methyltransferase gene omt - 1, and the internal tran- scribed spacer （ITS） of fungal 5.8S rDNA, a multiplex PCR reaction system for aflatoxigenic fungi was established and optimized, and then verified by detection of 10 testing stains. The results showed that this multiplex PCR method had many advantages, such as high sensitivity, strong specificity, convenient and efficient to operation. In conclusion, this method provided a key technical support for developing and applying of rapid detection kit of aflatoxigenic fungi.

关 键 词：多重PCR 产黄曲霉毒素真菌 快速检测 特异性 灵敏度 

分 类 号：Q93-31[生物学—微生物学]