20株鲍氏不动杆菌获得性耐药元件检测与菌株亲缘关系研究  

Acquired drug resistance genes and phylogenetic analysis of 20 strains of Acinetobacterbaumannii

作　　者：杨丽华[1] 陆利君[1] 吴毅凌[1] 翁幸鐾 糜祖煌[3] 

机构地区：[1]上海市松江区疾病预防控制中心微检科,上海201620 [2]宁波市第一人民医院检验科,浙江宁波315010 [3]无锡市克隆遗传技术研究所,江苏无锡214026

出　　处：《中华医院感染学杂志》2017年第2期259-262,共4页Chinese Journal of Nosocomiology

基　　金：上海市松江区医学重点学科建设项目(2013XK09)

摘　　要：目的了解鲍氏不动杆菌携带的获得性耐药元件,以及菌株间的亲缘关系。方法收集2015年1-12月医院分离到的20株鲍氏不动杆菌,采用聚合酶链反应(PCR)及序列分析的方法分析31种β-内酰胺类药物获得性耐药基因和12种氨基糖苷类药物获得性耐药基因以及8种可移动遗传元件遗传标记,并对检测结果作样本聚类分析(UPGMA法)。结果本组菌对11种抗菌药物均耐药(100.0%),共检出4种β-内酰胺类药物获得性耐药基因和5种氨基糖苷类药物获得性耐药基因以及5种可移动遗传元件遗传标记,且阳性率较高;每株均能检出β-内酰胺类药物与氨基糖苷类药物获得性耐药基因及可移动遗传元件遗传标记;耐药元件检测结果共分为6种阳性模式;样本聚类分析提示,20株鲍氏不动杆菌呈明显的聚集性,其中2-5-7-8-9-10-13-15-17-20号株(共10株),11-12-16-19号株(共4株),1-4-6号株(共3株)为克隆传播。结论本组菌携带的β-内酰胺类和氨基糖苷类耐药基因和耐药表型一一对应,携带的可移动遗传元件是获得性耐药基因快速传播的原因,特定耐药元件的检测是研究细菌耐药性的一种实用方法。OBJECTIVE To understand the acquired drug resistance genes in 20 strains of Acinetobacter baumannii and analyze the phylogenetic relationship among the strains. METHODS A total of 20 strains of A. baumannii were isolated from Jan 2015 to Dec 2015. Totally 31 kinds of acquired resistance genes to beta-lactams, 12 kinds of acquired resistance genes to aminoglycosides, and 8 kinds of mobile genetic elements were analyzed by means of pol- ymerase-chain-reaction （PCR） and sequencing analysis, and the sample cluster analysis （UPGMA） was performed for the detection result. RESULTS This groups of strains were resistant to all of the 11 kinds of antibiotics. Totally 4 kinds of acquired resistance genes to beta-lactams, 5 kinds of acquired resistance genes to aminoglycosides, and 5 kinds of mobile genetic elements were detected positive in the strains, and the positive rates were very high. In addition, the acquired resistance genes to beta-lactams and aminoglycosides, and mobile genetic elements could be detected positive in each of the strains. The detected resistance elements were divided into 6 positive modes. The sam- pie cluster analysis indicated that the 20 strains of A. baumannii presented with obvious aggregation, and strain No. 2-5-7-8-9-10-13-15-17-20 （10 strains）, strain No. 11-12-16-19 （4 strains）, and strain No. 1-4-6 （3 strains） were clonal propagation. CONCLUSION The phenotypes and genotypes of acquired resistance genes to beta-lactams and aminoglycosides are homologous in this group of A. baumannli, the mobile genetic elements are the causes of rapid propagation of acquired resistance genes, and the detection of specified resistance genes can be used as a practical method to study the bacterial resistance.

关 键 词：鲍氏不动杆菌 亲缘性分析 获得性耐药元件 Β-内酰胺类 氨基糖苷类 可移动遗传元件 

分 类 号：R378[医药卫生—病原生物学]