全血中铅的快速测定法  

Rapid Method for Determination of Lead in Whole Blood

作　　者：毕珊[1] 霍学义[1] 邬丽[1] 陈子良[1] 

机构地区：[1]贵州省疾病预防控制中心,贵阳550004

出　　处：《微量元素与健康研究》2017年第1期64-66,共3页Studies of Trace Elements and Health

摘　　要：目的:建立一种简便、快速、准确可靠的石墨炉原子吸收光谱法测定全血中铅的方法。方法:全血样经0.02%Pd Cl2+0.5%Triton X-100+0.1%HNO3混合基体改进剂稀释后直接进样,塞曼背景校正,峰面积定量,石墨炉原子吸收标准加入校正法进行测定。结果:血铅含量在0~200μg/L范围内线性关系良好,相关系数r=0.9996,方法的检出限为0.38μg/L,加标回收率为97.9%~104.2%,RSD为4.3%~5.5%,应用方法测定GBW(E)090033-090036全血铅、镉成分分析标准物质结果与标准值相吻合。结论:房法准确度、精密度良好,样品不需消化,污染小,是一种快速批量测定全血中铅较为理想的方法。Objective： To develop a simple,rapid and accurate method to determine lead in the whole blood with graphite furnace atomic absorption spectrometry. Methods： The whole blood sample was directly diluted by 0. 02% Pd Cl2＋ 0. 5% Triton X- 100 ＋ 0. 1%HNO3,and then determined by graphite furnace atomic absorption standard addition method via direct automatic injection,Zeeman effect was used for background correction and peak area for quantification. Results： The blood lead content was linear in the range of0μg / L ~ 200μg / L;correlation coefficient was 0. 9996; detection limit was 0. 38μg / L; the recovery of standard addition was during97. 9 % ~ 104. 2%; RSD were 4. 3% ~ 5. 5%. The method was successfully applied to determine Certified Reference Materials such as GBW（ E）090033- 090036,and the results were in good agreement with the certified values. Conclusion：This method is characterized by good accuracy and precision,no digestion process and little pollution. It is a rapid,in batch and ideal method for determination of lead in whole blood.

关 键 词：血铅 石墨炉原子吸收法 测定 

分 类 号：O657.31[理学—分析化学]