缩泉胶囊改善糖尿病小鼠膀胱功能  

Improvement of Suoquan Capsules on bladder function of diabetic mice

作　　者：范平龙 连大卫 徐伟君[1] 扶丽君 黄萍[1] 操红缨[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510405

出　　处：《中成药》2017年第1期1-6,共6页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金(81673676)

摘　　要：目的研究缩泉胶囊(乌药、益智仁、山药)对糖尿病小鼠膀胱病变的改善作用及其作用机制。方法 60只小鼠随机分为正常组(n=8)及建模组(n=52),后者采用高脂饲料喂养加腹腔注射链脲佐菌素(STZ)诱导糖尿病模型,将造模成功小鼠(n=32)随机分为模型组,甲钴胺片组,缩泉胶囊低、高剂量组。观察缩泉胶囊对糖尿病小鼠空腹血糖值(FBG)、糖化血清蛋白(GSP)水平及一般情况的影响;测定膀胱漏尿点压(BLPP),并对膀胱进行组织病理学染色;利用荧光双标法检测P物质及NK1受体表达。结果各组FBG、GSP、体质量、摄食量和饮水量无明显差异,高剂量缩泉胶囊可以显著降低小鼠尿量。与模型组相比,缩泉胶囊治疗后能显著升高BLPP、P物质、NK1受体表达,并能明显改善小鼠膀胱组织形态学。结论缩泉胶囊改善糖尿病小鼠膀胱病变,其机制可能与上调膀胱组织中P物质及NK1受体表达有关。AIM To investigate the improving effect of Suoquan Capsules（ Linderae Radix,Alpiniae oxyphyllae Fructus and Dioscoreae Rhizoma） on mice with diabetic cystopathy and its mechanism of action. METHODS Sixty mice were randomly assigned into normal group（ n = 8） and model group（ n = 52）; the diabetic models of the latter were induced by high-fat feeding combined with streptozotocin（ STZ） injection,then modeled mice（ n = 32） were randomly divided into model,Mecobalamin Tablets,low-and high-dose Suoquan Capsules groups.The influences of Suoquan Capsules on fasting blood glucose（ FBG）,glycated serum protein（ GSP） level and general conditions were observed. The bladder leak point pressure（ BLPP） was determined. Histopathological staining was performed on urinary bladder. And the expressions of substance P and NK1 receptor were detected by double immunofluorescent staining. RESULTS There were no significant differences in FBG,GSP,body weight,food intake and water consumption among various groups. The high-dose Suoquan Capsules significantly decreased urine volume of mice. Compared with the model group,the treatment with Suoquan Capsules markedly increased BLPP,the expressions of substance P and NK1 receptor were significantly increased,and the histopathology of bladder in mice was obviously improved. CONCLUSION Suoquan Capsules improves the diabetic cystopathy in mice,and its mechanism maybe related to the up-regulation of substance P and NK1 receptor expressions in bladder tissue.

关 键 词：缩泉胶囊 糖尿病膀胱病变 P物质 NK1受体 

分 类 号：R285.5[医药卫生—中药学]