艾纳香油对紫外线诱导小鼠皮肤晒伤的保护作用  

Protective effect of essential oils from Blumea balsamifera on sunburn induced by UVB in mouse skin

作　　者：李小婷[1,2,3] 庞玉新[1,2] 王丹[1,2] 杨全 范佐旺[1,2,3] 马青松[1,2,3] 许罗凤[1,2,3] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,海南儋州571737 [2]海南省艾纳香工程技术研究中心,海南儋州571737 [3]广东药科大学中药学院,广东广州510006

出　　处：《中成药》2017年第1期26-32,共7页Chinese Traditional Patent Medicine

基　　金：科技部科研院所研发专项资金项目(2013EG1343239);贵州省重大科技专项计划项目(2013-6011)

摘　　要：目的研究艾纳香油[Blumea balsamifera(L.)DC.,BBO]对紫外线诱导小鼠皮肤晒伤的保护作用及其作用机制。方法采用急性紫外线UVB辐射小鼠皮肤建立晒伤模型,创面外用艾纳香油,HE染色观察晒伤皮肤组织的病理变化,测定超氧化物岐化酶(SOD)活性及丙二醛(MDA)、还原型谷胱甘肽(GSH)含有量,利用酶联免疫吸附法(ELISA)检测表皮中8-羟基脱氧鸟嘌呤核苷(8-OHd G)、白细胞介素6(IL-6)及核转录因子-Kappa B(NF-κB)水平,免疫组化法检测增殖细胞核抗原(PCNA)、P53肿瘤抑制蛋白、肿瘤坏死因子(TNF-α)蛋白表达。结果与模型组比较,艾纳香油显著减少表皮厚度,使小鼠皮肤中SOD活性、GSH和MDA含有量得到恢复,显著降低8-OHd G、IL-6和NF-κB水平,同时抑制P53、PCNA表达。结论艾纳香油能够缓解UVB引起的皮肤晒伤,其机制与增强抗氧化作用,抑制NF-κB信号通路,下调IL-6释放,减少8-OHd G和PCNA水平有关。AIM To study the protective effect of essential oils from Blumea balsamifera（ L.） DC.（ BBO）on UVB-induced sunburn in mouse skin and its mechanism of action. METHODS The model for sunburned mouse skin was established by acute UVB irradiation. Essential oils from B. balsamifera were applied to the surface of wound for external use. The pathological changes of sunburned skin tissue were observed by hematoxylin-eosin（ HE） staining. The activity of superoxide dismutase（ SOD）,and the contents of malondialdehyde（ MDA） and glutathione（ GSH） were measured. The levels of 8-hydroxy-desoxyguanosine（ 8-OHd G）,interleukin-6（ IL-6）and nuclear factor kappa-B（ NF-κB） in epidermis were detected by ELISA. Additionally,the expressions of tumor necrosis factor-alpha（ TNF-α）,P53 tumor suppressor protein and proliferation cell nuclear antigen（ PCNA） were evaluated by immunohistochemistry（ IHC）. RESULTS Compared with the model group,treatment with essential oils from B. balsamifera significantly reduced the thickness of epidermis,and the activity of SOD and the contents of MDA,GSH in mouse skin were restored. In addition,the essential oils from B. balsamifera resulted in a significant decrease in levels of 8-OHd G,IL-6 and NF-κB,and an inhibition in expressions of P53 and PCNA.CONCLUSION The essential oils from B. balsamifera can alleviate UVB-induced sunburn. Its mechanism is related to enhanced antioxidant power,inhibited NF-κB signal passway,down-regulated release of IL-6 and reduced levels of 8-OHd G,PCNA.

关 键 词：艾纳香油 晒伤 NF-ΚB P53 PCNA 

分 类 号：R285.5[医药卫生—中药学]